Shifa Ahmad, P K Hashim, Masamichi Imajo, Nusaiba Madappuram Cheruthu, Kiyonori Takahashi, Shinya Tanaka, Takayoshi Nakamura, Nobuyuki Tamaoki
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引用次数: 0
Abstract
Based on the known Wnt agonist BML-284, we designed and synthesized photoswitchable azo derivative compounds that can act as agonists for the Wnt signaling pathway. These photoswitchable agonists were shown to undergo reversible trans-cis isomerization upon being irradiated with visible light, but only the cis isomer was observed to activate the Wnt signaling pathway, using a luminescense-based reporter assay in cultured cells. One of the compounds, denoted as compound 2, showed ∼88% agonist activity after being subjected to visible light irradiation in comparison to the non-photoswitchable BML-284. We also were able to selectively activate the Wnt signaling pathway using 2 and light irradiation at a specific region of interest in a model cell culture system, highlighting the ability to achieve spatiotemporal regulation.
期刊介绍：
Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.
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