Efficient genetic transformation and genome editing via an Agrobacterium-mediated in commercial oat (Avena sativa L.) cultivars

IF 9.3 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Integrative Plant Biology Pub Date : 2025-04-07 DOI:10.1111/jipb.13915

Kun Shi, Weihong Huang, Mengxin Zhu, Shouzhen Teng, Jinli Zhang, Zhizhen Duan, Chenchen Zhu, Tao Hu, Ke Wang, Zan Wang

引用次数: 0

Abstract

An optimized Agrobacterium-mediated transformation protocol for immature and mature oat embryos increased transformation efficiencies and the number of transformable cultivars and enabled highly efficient CRISPR/Cas9 and CRISPR/Cas12i genome editing to accelerate oat biotechnology breeding.

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农杆菌介导的商业燕麦品种高效遗传转化和基因组编辑

优化的农杆菌介导的未成熟和成熟燕麦胚胎转化方案提高了转化效率和可转化品种的数量，并实现了高效的CRISPR/Cas9和CRISPR/Cas12i基因组编辑，加速了燕麦生物技术育种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Integrative Plant Biology 生物-生化与分子生物学

CiteScore

18.00

自引率

5.30%

发文量

220

审稿时长

3 months

期刊介绍： Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.