SIRT5 alleviates oxidative stress of boar sperm induced by cryopreservation through IDH2 and SOD2 pathway

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-04-05 DOI:10.1016/j.theriogenology.2025.117424

Malik Ahsan Ali , Yan Zhang , Qingyong Ni , An-qi Huang , Shan Dou , Yiguo Tang , Xinhong Xu , Changjun Zeng

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Abstract

Excessive production of reactive oxygen species (ROS) and lipid peroxidation result in decreased motility, fertility, farrowing rate and litter size during boar sperm cryopreservation. Our previous study reported that cryopreservation leads to differential expression of genes (SIRT5 and IDH2) which regulate the energy metabolism and quality of sperm. But how SIRT5 regulates the metabolism and oxidative stress in boar sperm are still unknown. In the present study, we first determined the regulation of IDH2 and SOD2 by SIRT5 after its knockdown and then determined the role of SIRT5 in regulation of oxidative stress, metabolic activities, motility and fertility potential by its specific inhibitor (Suramin, SUR) and activator (Resveratrol, RSV). Then the effect of cryopreservation was evaluated on mRNA and protein expressions of SIRT5, IDH2 and SOD2 in boar sperm. A decrease in expressions was observed at both mRNA and protein for all these genes. Additionally, RSV and SUR treatment led to up and downregulation of mRNA and protein of both IDH2 and SOD2. A significant decrease (P < 0.05) in ROS levels and improvement in sperm motility, acrosomal integrity, MMP, PMI and ATP were observed in post-thaw sperm after activation of SIRT5 with RSV. Additionally, BAX and 4HNE levels were significantly (P < 0.05) reduced in RSV treated sperm. In conclusion, this study demonstrated that SIRT5 activator (RSV) improved the quality of boar sperm by upregulation of IDH2 and SOD2. At the same time, the oxidative stress was reduced via interaction among SIRT5, IDH2 and SOD2.

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SIRT5通过IDH2和SOD2途径缓解猪精子低温保存引起的氧化应激

在猪精子冷冻保存过程中，活性氧（ROS）和脂质过氧化的过量产生导致精子活力、生育能力、产仔率和产仔数下降。我们之前的研究报道了冷冻保存导致调节精子能量代谢和质量的基因（SIRT5和IDH2）的差异表达。但是SIRT5如何调节猪精子的代谢和氧化应激仍然未知。在本研究中，我们首先确定了SIRT5敲低后对IDH2和SOD2的调控作用，然后确定了SIRT5通过其特异性抑制剂（苏拉明，SUR）和激活剂（白藜芦醇，RSV）调控氧化应激、代谢活性、运动能力和生育潜力的作用。然后观察低温保存对猪精子中SIRT5、IDH2和SOD2 mRNA和蛋白表达的影响。所有这些基因的mRNA和蛋白表达均下降。此外，RSV和SUR处理导致IDH2和SOD2 mRNA和蛋白的上调和下调。显著下降(P <；RSV激活SIRT5后，解冻后精子中ROS水平升高，精子活力、顶体完整性、MMP、PMI和ATP均有改善。此外，BAX和4HNE水平显著(P <；0.05)。综上所述，本研究表明SIRT5激活剂（RSV）通过上调IDH2和SOD2来改善猪精子质量。同时，SIRT5、IDH2和SOD2相互作用降低了氧化应激。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.