Multiparametric analysis of tumor infiltrating lymphocytes in solid tumors.

4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2023-07-22 DOI:10.1016/bs.mcb.2023.03.006

Rebecca Borella, Annamaria Paolini, Beatrice Aramini, Lara Gibellini, Valentina Masciale, Domenico Lo Tartaro, Massimo Dominici, Sara De Biasi, Andrea Cossarizza

引用次数: 0

Abstract

The use of single-cell technologies in characterizing the interactions between immune and cancer cells is in continuous expansion. Indeed, the combination of different single-cell approaches enables the definition of novel phenotypic and functional aspects of the immune cells infiltrating the tumor microenvironment (TME). This approach is promoting the discovery of relevant and reliable predictive biomarkers, along with the development of new promising treatments. In this chapter, we describe the main subsets of tumor-infiltrating lymphocytes from a phenotypical and functional point of view and discuss the use of single-cell technologies used to characterize these cell populations within TME.

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单细胞技术在描述免疫细胞和癌细胞之间相互作用特征方面的应用正在不断扩大。事实上，将不同的单细胞方法结合起来，可以确定浸润肿瘤微环境（TME）的免疫细胞的新表型和功能。这种方法有助于发现相关可靠的预测性生物标志物，并开发出前景广阔的新疗法。在本章中，我们将从表型和功能的角度描述肿瘤浸润淋巴细胞的主要亚群，并讨论如何利用单细胞技术表征 TME 中的这些细胞群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in cell biology 生物-细胞生物学

CiteScore

3.10

自引率

0.00%

发文量

125

审稿时长

3 months

期刊介绍： For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.