Effects of ovariectomy-induced osteoporosis and two photobiomodulation protocols on the repair of apical periodontitis in Wistar rats: A microCT study

IF 7.1 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International endodontic journal Pub Date : 2025-04-04 DOI:10.1111/iej.14234

Carolina Horn Troian-Michel, Bruna Barcelos Só, Lauren Frenzel Schuch, Grazielle Oliveira Stelter, Tuany Rafaeli Schmidt, Joana Letícia Schorr, Daniela Campagnol, Tuane Nerissa Alves Garcez, Gabriel Barcelos Só, Thalita Ayres Arrué, Theodoro Weissheimer, Manoela Domingues Martins, Marcus Vinicius Reis Só

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引用次数: 0

Abstract

Aim

Osteoporosis is a disease that is related to a higher prevalence and greater volumes of apical periodontitis. The purpose of this study was to evaluate the effect of ovariectomy-induced osteoporosis and high- or low-level laser photobiomodulation (PBM) on the repair of apical periodontitis (AP) in adult female Wistar rats.

Methodology

Sixty female Wistar rats (n = 120) were randomly allocated into four control (n = 6 teeth) and 8 experimental (n = 12 teeth) groups, according to procedure and time of evaluation: healthy control (GCS14/GCS28), osteoporotic control (GCO14/GCO28), sham (SHAM14/SHAM28), osteoporotic (OVX14/OVX28), low-level laser (OVX-LLL14/OVX-LLL28) and high-level laser (OVX-GEM14/OVX-GEM28). All groups were submitted to bilateral ovariectomy, except for the healthy control and sham. Eight weeks later, AP was induced bilaterally in mandibular first molars' mesial roots in experimental groups, and after 21 days, their endodontic treatment (RCT) was conducted. For 14 or 28 days, PBM was applied three times/week, as follows: sham application (SHAM and OVX groups); low-level laser (OVX/LLL groups); defocused high-power laser (OVX/GEM groups). After the respective experimental periods, the animals were euthanized. Tibias and hemi-mandibula were obtained and submitted to computed microtomography. Bone volume fraction (BV/TV), trabeculae number (Tb.N), spacing (Tb.Sp) and thickness (Tb.Th) were obtained for tibias, while RCT apical limit, periodontal ligament and AP volumes (APvol) were recorded for hemi-mandibula. Data were subjected to statistical analysis through generalized estimating equations (GEE), ANOVA and chi-square tests (5% significance level).

Results

Ovariectomy tibias showed lower BV/TV and Tb.N (p < .0001) and higher Tb.Th (p = .020) and Tb.Sp (p < .0001) when compared to sham groups. For hemi-mandibula, lower APvol values were found in SHAM14 when compared to OVX/LLL14 and OVX/GEM14 groups (p < .05), which were not different from each other. Over the 28-day period, the OVX/LLL28 group showed higher AP volumes when compared to OVX28 and OVX/GEM28 (p < .05). Analysing variables effects on APvol, time, PBM and RCT apical limit showed no effect (p > .05), while ovariectomy showed a significant increase (p = .004).

Conclusions

Ovariectomy-induced osteoporosis in Wistar rats led to a reduction in AP repair, and PBM was unable to counteract this effect.

Abstract Image

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显微ct研究卵巢切除所致骨质疏松和两种光生物调节方案对Wistar大鼠根尖牙周炎修复的影响。

目的：骨质疏松症是一种与根尖牙周炎的高发病率和高发病量有关的疾病。本研究的目的是评估卵巢切除诱导的骨质疏松症和高或低水平激光光生物调节（PBM）对成年雌性 Wistar 大鼠根尖牙周炎（AP）修复的影响：将 60 只雌性 Wistar 大鼠（n = 120）按评估程序和时间随机分为 4 个对照组（n = 6 颗牙齿）和 8 个实验组（n = 12 颗牙齿）：健康对照组（GCS14/GCS28）、骨质疏松对照组（GCO14/GCO28）、假阳性组（SHAM14/SHAM28）、骨质疏松组（OVX14/OVX28）、低强度激光组（OVX-LLL14/OVX-LLL28）和高强度激光组（OVX-GEM14/OVX-GEM28）。除健康对照组和假组外，所有组均接受双侧卵巢切除术。八周后，在实验组下颌第一磨牙的中根诱导双侧AP，21天后进行牙髓治疗（RCT）。在 14 天或 28 天内，每周应用三次 PBM，具体如下：假应用（SHAM 和 OVX 组）；低水平激光（OVX/LLL 组）；散焦高功率激光（OVX/GEM 组）。实验结束后，动物被安乐死。获取胫骨和半胫骨并进行计算机显微断层扫描。获得胫骨的骨体积分数（BV/TV）、骨小梁数（Tb.N）、间距（Tb.Sp）和厚度（Tb.Th），记录半胫骨的RCT顶端界限、牙周韧带和AP体积（APvol）。通过广义估计方程（GEE）、方差分析和卡方检验（5%显著性水平）对数据进行统计分析：结果：卵巢切除术后的胫骨BV/TV和Tb.N较低（p .05），而卵巢切除术后的胫骨BV/TV和Tb.N显著增加（p = .004）：结论：卵巢切除术引起的 Wistar 大鼠骨质疏松症导致 AP 修复能力下降，而 PBM 无法抵消这种影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International endodontic journal 医学-牙科与口腔外科

CiteScore

10.20

自引率

28.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.