Plasmon-enhanced fluorescence sensor based on Au nanocages for sensitive detection of norepinephrine

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-04-01 DOI:10.1016/j.aca.2025.343995

Chenye Xu, Shilin Chen, Wen Sun, Rong Xu, Xiangyuan Yin, Dongsheng Guo, Yaqiong Yang

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Abstract

Background

Norepinephrine (NE) as a crucial monoamine neurotransmitter in the central and sympathetic nervous system, plays an important role in different physiological and pathophysiological processes. Brain NE can modulate cerebrospinal fluid flux and neurovascular coupling, regulate cortical and hippocampal neuronal circuitry, and participate the immune system. In addition, the reduced concentration of NE in brain was currently deemed to be the internal reason of major depression. However, development of detection method of NE with high spatiotemporal resolution in living systems remains a great challenge.

Results

Herein, a plasmon-enhanced fluorescence (PEF) sensor based on Au nanocages (Aucages) were designed and prepared for ultra-sensitive detection of NE. Aucages with porous walls, hollow interior and systematically tunable localized surface plasmon resonance (LSPR) wavelengths (536 nm, 654 nm, 754 nm) were prepared to obtain a highly fluorescent enhancement of Au nanoclusters (Au NCs). Moreover, polyethylene glycol (PEG) with different molecular weight (1000, 5000, 10000 Da) were applied to control the distance between the Aucages and Au NCs. 3D-FDTD simulation results indicated that the fluorescence enhancement was primarily due to the internal and external enhanced electric field effects of Aucages. This sensor was applied for the turn-on detection of NE in commonly used clinical injectable norepinephrine bitartrate with the recovery rate of 98.06-105.34%. Meanwhile, real-time fluorescence imaging of NE at living pheochromocytoma (PC-12) cells was explored with a red-emitted fluorescence.

Significance

This study first employed Aucages with more “hot spot” for red-emitted Au NCs to realize fluorescence enhancement. It provides a new method for the development of more sensitive, accurate and convenient analysis of NE in clinical drug analysis, cell monitor and metabolism study.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.