Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BMC Genomics Pub Date : 2025-03-28 DOI:10.1186/s12864-025-11500-5

Xuliang Luo, Yan Guo, Xuelian Li, Zi Mei, Haobo Zhou, Ping Qiu, Haoxin Wang, Yan Chen, Yanzhang Gong

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引用次数: 0

Abstract

Background: Aromatase, encoded by Cyp19a1, is the rate limiting enzyme in biosynthesis of estrogens, and excessive aromatase can reduce the semen quality in roosters. Seminal plasma extracellular vesicles (SPEV) are nanoscale vesicles that carry and transmit signaling molecules, thereby affecting semen quality. Currently it is still unclear whether SPEV are involved in the process of that aromatase affects the quality semen in chicken. To clarify this issue, lentivirus carrying Cyp19a1 (LV-CYP19A1) for over-expression of aromatase was constructed and injected to testis of 35-week-old roosters. Semen quality and seminal plasma hormone were measured, and SPEV were also extracted and proteome sequencing was performed after treatment of LV-CYP19A1.

Results: The results indicated that semen volume, fertility, sperm motility, testosterone (T) levels were significantly decreased, and estradiol (E₂) levels were significantly increased in LV-CYP19A1 group than those in control group (P < 0.05). Through proteomic analysis of SPEV, we identified 966 differentially expressed proteins (DEPs) in the comparison of LV-CYP19A1 group and control group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) items of DEPs are mainly enriched in ATP synthesis coupled electron transport, flagellated sperm motility, regulation of steroid biosynthetic process, and PI3K-Akt signaling pathway. Furthermore, 8 proteins including ENO4, APOB, SDHA, SDHB, UQCRC1, VIN, PITGB3 and FXN were identified as key proteins in SPEV involving in the process of aromatase regulated rooster semen quality.

Conclusions: Our results reveal that aromatase can down-regulate the protein expression related to regulation of ATP synthesis and metabolism, and sperm motility in SPEV, thereby reducing semen quality in roosters.

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芳香酶通过下调精浆细胞外囊泡中ATP合成相关蛋白的表达来降低精子活力。

背景：由Cyp19a1编码的芳香酶是雌激素生物合成的限速酶，过量的芳香酶会降低公鸡的精液质量。精浆细胞外囊泡（SPEV）是一种携带和传递信号分子的纳米级囊泡，从而影响精液质量。目前尚不清楚SPEV是否参与芳香化酶影响鸡精液质量的过程。为了澄清这一问题，构建了携带Cyp19a1 （LV-CYP19A1）过表达芳香化酶的慢病毒，并将其注射到35周龄公鸡的睾丸中。检测LV-CYP19A1治疗后的精液质量和精浆激素，提取SPEV并进行蛋白质组测序。结果：与对照组相比，LV-CYP19A1组公鸡精液量、生殖力、精子活力、睾酮(T)水平显著降低，雌二醇（E2）水平显著升高(P)。结论：我们的研究结果表明，芳香化酶可以下调SPEV中ATP合成和代谢调节相关蛋白的表达，降低精子活力，从而降低公鸡精液质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.