A pyrene-based fluorescent probe for cysteine detection in the presence of other biothiols and its application in living cells

IF 4 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Structure Pub Date : 2025-03-24 DOI:10.1016/j.molstruc.2025.142095

Mengzhou Wang, Jiayao Du, Yuefeng Ye, Guangzhou Lin, Chengyuan Liang, Wei Liu

引用次数: 0

Abstract

A novel fluorescent probe (E)-3-(pyrazin-2-yl)-1-(pyren-1-yl)prop‑2-en-1-one, (PPP), which pyrene structure attached to pyrazine derivative, was designed for detection of cysteine (Cys) since it plays an important role in many physiological and pathological processes. The results indicated that probe PPP could monitor Cys in the presence of other biothiols such as glutathione (GSH) and homocysteine (Hcy) under mild conditions. Furthermore, the probe PPP was successfully applied for cellular imaging and specific fluorescence detection of Cys in TE-1 cells with negligible cytotoxicity, which also helps to further understand the pathological process of related diseases.

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来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.