Redox-Regulated Cell Response Mechanisms to Nitrosyl Iron Complex with N-Ethylthiourea in Human Mesenchymal Stem Cells In Vitro.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-28 DOI:10.1007/s10517-025-06367-1

A A Balakina, V I Amozova, T S Stupina, N A Sanina

引用次数: 0

Abstract

We studied the processes of cell death and intracellular accumulation of free radicals, as well as activity of redox-sensitive transcription factors Nrf2 and NF-κB under the action of nitrosyl iron complex with N-ethylthiourea (ETU) on a model of human mesenchymal stem cells in vitro. ETU complex exhibits a pronounced prooxidant and cytotoxic effects accompanied by enhanced intracellular accumulation of nitrogen monoxide, induction of apoptotic cell death, activation of Nrf2/HO-1 pathways, and suppression of proinflammatory NF-κB-mediated reactions. A significant role of redox-dependent cellular processes in the mechanisms of the cytotoxic effect of nitrosyl iron complexes is shown.

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体外人间充质干细胞对n -乙基硫脲配合物亚硝基铁的氧化还原调控细胞反应机制

在体外培养的人间充质干细胞模型上，研究了n -乙基硫脲（ETU）配合亚硝基铁对细胞死亡和细胞内自由基积累的影响，以及氧化还原敏感转录因子Nrf2和NF-κB的活性。ETU复合物表现出明显的促氧化和细胞毒性作用，并伴有细胞内一氧化氮积累增强，诱导凋亡细胞死亡，激活Nrf2/HO-1通路，抑制NF-κ b介导的促炎反应。氧化还原依赖的细胞过程在亚硝基铁配合物的细胞毒性作用机制中的重要作用显示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.