Distribution and Genetic Characteristics of Seoul Virus in Different Organs of Rattus norvegicus.

IF 3.8 3区医学 Q2 VIROLOGY

Viruses-Basel Pub Date : 2025-03-14 DOI:10.3390/v17030412

Yamei Wei, Xiaodong Shi, Yanan Cai, Zhanying Han, Yanbo Zhang, Yonggang Xu, Xu Han, Qi Li

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Abstract

To investigate the distribution of hantavirus (HV) in rodent organs, we selected eight counties across four regions in Hebei Province (southern, northern, eastern, and central) as study areas. Rodents were captured using night trapping methods, and organ samples were aseptically collected for HV detection via quantitative real-time PCR (qPCR) and gene sequencing. During the 2022-2023 spring and autumn seasons, 1386 rodents were trapped, including 73 Rattus norvegicus carrying Seoul virus (SEOV). The highest detection rate was observed in the liver (3.84%), followed by the kidneys (3.46%) and lungs (3.09%). Viral load analysis revealed higher SEOV RNA levels in the liver than in the lungs and kidneys. Antibody levels in R. norvegicus may influence the detection of viruses in organs. Phylogenetic analysis indicated that all sequences belonged to the S3 subtype, exhibiting regional aggregation and genetic stability. Our findings emphasize the necessity of multi-organ sampling for comprehensive HV surveillance and epidemic risk assessment.

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汉城病毒在褐家鼠不同器官中的分布及遗传特征。

为了了解汉坦病毒（HV）在啮齿动物器官中的分布情况，我们选择了河北省南部、北部、东部和中部4个地区的8个县作为研究区。采用夹夜法捕获鼠类，无菌采集脏器标本，采用实时荧光定量PCR （qPCR）和基因测序进行HV检测。2022-2023年春秋季共捕获鼠类1386只，其中携带首尔病毒褐家鼠73只。检出率以肝脏最高（3.84%），其次为肾脏（3.46%）和肺部（3.09%）。病毒载量分析显示肝脏中的SEOV RNA水平高于肺和肾脏。褐家鼠体内抗体水平可能影响器官中病毒的检测。系统发育分析表明，所有序列均属于S3亚型，具有区域聚集性和遗传稳定性。我们的研究结果强调了多器官采样对全面的HV监测和流行风险评估的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.