Synthesis and evaluation of 177Lu-labeled anti-EGFR Fab antibody for lung cancer

IF 1.5 3区化学 Q3 CHEMISTRY, ANALYTICAL

Journal of Radioanalytical and Nuclear Chemistry Pub Date : 2025-03-04 DOI:10.1007/s10967-025-10030-4

Yuan-Yuan Li, Jia-Yan Yu, Tao Wang, Jing Wang, Peng Zhao, Xia Yang, Hong-Yuan Wei, Yue Chen

引用次数: 0

Abstract

Fab fragments obtained via enzymatic digestion of full-length antibodies or by gene recombination technology have smaller molecular weight and greater penetration ability. Those advantages make it uniquely suited to deliver radionuclides. In this study, we prepared a Fab fragment from the anti-EGFR antibody Cetuximab and labeled it with ¹⁷⁷Lu. The binding activity in vitro and biodistribution in vivo of [¹⁷⁷Lu]Lu-DOTA-Fab-Cetuximab were investigated. The [¹⁷⁷Lu]Lu-labeled Fab fragment showed improved tumor penetration and faster blood clearance compared to the full-length antibody.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Radioanalytical and Nuclear Chemistry 化学-分析化学

CiteScore

2.80

自引率

18.80%

发文量

504

审稿时长

2.2 months

期刊介绍： An international periodical publishing original papers, letters, review papers and short communications on nuclear chemistry. The subjects covered include: Nuclear chemistry, Radiochemistry, Radiation chemistry, Radiobiological chemistry, Environmental radiochemistry, Production and control of radioisotopes and labelled compounds, Nuclear power plant chemistry, Nuclear fuel chemistry, Radioanalytical chemistry, Radiation detection and measurement, Nuclear instrumentation and automation, etc.