Microgels based on carboxymethylcellulose as multifunctional carriers for immobilization of inhibitor and activator of inducible NO synthase

Abstract

Three-component (ternary) microgels based on carboxymethylcellulose and copper(ii) ions with the immobilized inhibitor of inducible NO synthase (N-(5,6-dihydro-4H-1,3-thiazin-2-yl)benzamide), as well as the activator of this enzyme (N-(4-isopropylphenyl)-N-(1-iminoethyl)piperidine-1-carbothioamide hydrobromide) were prepared. The hydrodynamic and electrokinetic characteristics of the microgels were studied and demonstrate an optimal size and high colloidal stability in both aqueous and physiological environments. The synthesized ternary microgels are characterized by high cytotoxicity causing apoptosis of leukemic Jurkat cells.