{"title":"Current issues of tandem mass spectrum (MS2)-based glycoproteomics and efforts to complement them","authors":"Hiroyuki Kaji","doi":"10.1016/j.bbadva.2025.100158","DOIUrl":null,"url":null,"abstract":"<div><div>With the development of liquid chromatography/mass spectrometers that support proteomics and the associated development of analytical methods and data analysis software, the number of proteins that can be identified and quantified in a single analysis is approaching the level of transcriptomics. However, many problems remain to be solved in protein glycosylation analysis. This mini-review discusses the technical issues of MS2-based glycoprotein identification and efforts to complement them by the Human Glycome Atlas (HGA) Project in Japan.</div></div>","PeriodicalId":34672,"journal":{"name":"BBA Advances","volume":"7 ","pages":"Article 100158"},"PeriodicalIF":3.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BBA Advances","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667160325000213","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
With the development of liquid chromatography/mass spectrometers that support proteomics and the associated development of analytical methods and data analysis software, the number of proteins that can be identified and quantified in a single analysis is approaching the level of transcriptomics. However, many problems remain to be solved in protein glycosylation analysis. This mini-review discusses the technical issues of MS2-based glycoprotein identification and efforts to complement them by the Human Glycome Atlas (HGA) Project in Japan.