Positive regulation of BBX11 by NAC053 confers stomatal and apoplastic immunity against bacterial infection in Arabidopsis

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-03-20 DOI:10.1111/nph.70096

Sheng Luo, Charles Tetteh, Zhiqiang Song, Cheng Zhang, Pinyuan Jin, Xingqian Hao, Yingjun Liu, Shating Ge, Jiao Chen, Keke Ye, Kang Wang, Ting Zhang, Huajian Zhang

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引用次数: 0

Abstract

Stomatal immunity and apoplastic immunity are critical for preventing microbial phytopathogenesis. However, the specific regulatory mechanisms of these resistances remain unclear.
In this study, a BBX11 transcription factor (TF) was identified in Arabidopsis and was found to participate in stomatal and apoplast immunity. Phenotypic, biochemical, and genetic analyses revealed that NAC053 contributed to Arabidopsis resistance against Pseudomonas syringae pv tomato DC3000 (Pst DC3000) by positively regulating BBX11.
BBX11 TF that was expressed constitutively in guard cells acts as a positive regulator of plant defense against Pst DC3000 through the suppression of coronatine (COR)-induced stomatal reopening, mitigating the virulence of COR and alleviating COR-triggered systemic susceptibility in the apoplast. BBX11 was found to be involved in PTI responses induced by flg22, such as stomatal closure, reactive oxygen species accumulation, MAPK activation, and callose deposition, thereby enhancing disease resistance. Yeast one-hybrid screening identified NAC053 as a potential TF that interacted with the promoter of BBX11. NAC053 also positively regulated resistance to Pst DC3000.
These findings underscore the significance of transcriptional activation of BBX11 by NAC053 in stomatal and apoplastic immunity against Pst DC3000, enhancing understanding of plant regulatory mechanisms in response to bacterial pathogens.

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NAC053对BBX11的正调控赋予拟南芥气孔和外胞体对细菌感染的免疫

气孔免疫和胞外免疫是预防微生物植物发病的关键。然而，这些耐药性的具体调控机制尚不清楚。本研究在拟南芥中发现了一个BBX11转录因子（TF），该转录因子参与了气孔和外质体免疫。表型、生化和遗传分析表明，NAC053通过正调控BBX11参与拟南芥对丁香假单胞菌pv番茄DC3000 （Pst DC3000）的抗性。BBX11 TF在保护细胞中组成性表达，通过抑制coronatine （COR）诱导的气孔重新打开，减轻COR的毒力，减轻COR在外质体中引发的全身易感性，作为植物防御Pst DC3000的正调节因子。BBX11参与flg22诱导的PTI反应，如气孔关闭、活性氧积累、MAPK激活和胼胝质沉积，从而增强抗病性。酵母单杂交筛选发现NAC053是与BBX11启动子相互作用的潜在TF。NAC053还积极调节对Pst DC3000的抗性。这些发现强调了NAC053转录激活BBX11在Pst DC3000的气孔和外胞免疫中的重要意义，增强了对植物对细菌病原体的调节机制的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.