A novel red-emitting fluorescent probe reveals the depletion of vicinal dithiol-containing proteins in acute alcoholic liver injury (AALI) mice model

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI:10.1016/j.snb.2025.137639

Zahid Nasim Sayed , Ruipeng Shen , Jirong Liu , Qiyu Chen , Haijuan Zhang , Jingyi Liu , Alsiddig Osama , Shah Fahad , Baoxin Zhang , Jianguo Fang

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Abstract

Vicinal dithiol-containing proteins (VDPs) are a special type of redox-active thiol-carrying ubiquitous proteins with essential role in maintaining redox balance. The abnormal cellular levels of VDPs are known to be implicated in numerous pathological conditions including cancer, diabetes, stroke and neurodegenerative diseases. Therefore, developing small molecule fluorescent tools for the sensitive and selective detection of VDPs is indispensable. Herein, we rationally designed and screened a red-emitting fluorescent probe (FRP-6) carrying α, β-unsaturated acrylamide as a receptor unit, for the precise detection of VDPs. The probe demonstrated excellent capability to identify its target both in the living cells and in vivo without being interfered by other biologically relevant analytes. Additionally, FRP-6 was employed to detect the depletion of VDPs levels in drug induced liver injury (DILI) and acute alcoholic liver injury (AALI) models.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.