SARS-CoV-2 Entry Factors ACE2, TMPRSS2 and FURIN in Salivary Glands From Primary Sjögren's Disease

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2025-03-17 DOI:10.1111/jop.13621

Fernanda Aragão Felix, Flávia Martins Vasconcelos Filiú, Tarcília Aparecida da Silva, Débora Cerqueira Calderaro, Leandro Augusto Tanure, Maurício Augusto Aquino de Castro, Ricardo Santiago Gomez, Marina Gonçalves Diniz, Sílvia Ferreira de Sousa

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引用次数: 0

Abstract

Background

Salivary glands are the reservoir for severe acute respiratory syndrome Coronavirus 2 virus (SARS-CoV-2). It is known that SARS-CoV-2 uses angiotensin-converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2) and FURIN to infect cells. Salivary glands express these entry factors, and it is unclear if these molecules are expressed in salivary glands from primary Sjögren's disease (pSjD). This study assessed ACE2, TMPRSS2, and FURIN gene expression by RT-qPCR in pSjD compared to normal salivary gland and pleomorphic adenoma.

Methods

An integrated study of ACE2, TMPRSS2, and FURIN gene expression across pSjD, pleomorphic adenoma, and normal salivary gland by RT-pPCR was performed. The association between gene expression and clinicopathological data was also examined.

Results

pSjD showed ACE2 upregulation compared with normal salivary gland and pleomorphic adenoma (p < 0.05). TMPRSS2 and FURIN mRNA levels are also higher in pSjD compared to pleomorphic adenoma (p < 0.05). ACE2 expression levels were associated with anti-SSA/SSB positivity (p < 0.05). A strong correlation was observed between TMPRSS2 and FURIN in pSjD (r = 0.768; p = 0.001).

Conclusion

Our analysis revealed a high expression of ACE2 in pSjD. ACE2, TMPRSS2, and FURIN mRNA levels are increased in pSjD in comparison to pleomorphic adenomas. Neither ACE2, TMPRSS2, nor furin was consistently associated with SARS-CoV-2 positivity in pSjD. These findings may guide future investigations on the effects of SARS-CoV-2 in a subset of salivary gland diseases.

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原发性Sjögren病唾液腺中SARS-CoV-2进入因子ACE2、TMPRSS2和FURIN

背景：唾液腺是严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）的宿主。已知SARS-CoV-2利用血管紧张素转换酶2 （ACE2）、跨膜丝氨酸蛋白酶2 （TMPRSS2）和FURIN感染细胞。唾液腺表达这些进入因子，目前尚不清楚这些分子是否在原发性Sjögren病（pSjD）的唾液腺中表达。本研究通过RT-qPCR检测了pSjD中ACE2、TMPRSS2和FURIN基因的表达，并与正常唾液腺和多形性腺瘤进行了比较。方法：采用RT-pPCR方法对pSjD、多形性腺瘤和正常唾液腺中ACE2、TMPRSS2和FURIN基因的表达进行综合研究。基因表达与临床病理数据之间的关系也被检查。结果：与正常涎腺和多形性腺瘤相比，pSjD中ACE2表达上调(p)。与多形性腺瘤相比，pSjD中ACE2、TMPRSS2和FURIN mRNA水平升高。ACE2、TMPRSS2和furin与pSjD中SARS-CoV-2阳性均不一致。这些发现可能指导未来对SARS-CoV-2在一部分唾液腺疾病中的作用的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.