Transcriptome analysis and molecular docking reveal the activation of FOXO4, TNFSF15 and CASP9 in HeLa cells treated with DCM fraction from Clinacanthus nutans (C. nutans).

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2025-03-17 DOI:10.1080/10286020.2025.2469691

Kristine Sandra Pey Adum, Nor Hasyimah Haron, Zaleha Md Toha, Hasni Arsad

引用次数: 0

Abstract

Clinacanthus nutans (C. nutans) is a well-known herb in tropical Asia. Previous studies have reported the anticancer activity of C. nutans but the molecular mechanisms on cervical cancer are not fully understood. Therefore, our study aims to explore its effects on HeLa line and analyse the molecular interactions. Firstly, the RNA-Seq reads were processed for differentially expressed analyses. With Ingenuity Pathway Analysis (IPA) method, five cell death-related pathways with three significant genes (CASP9, FOXO4, TNFSF15) were identified. This study provides insight into the potential role of the DCM fraction of C. nutans mediating cell death mechanism in cervical cancer.

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转录组分析和分子对接显示，在用Clinacanthus nutans (C. nutans) DCM提取物处理HeLa细胞后，FOXO4、TNFSF15和CASP9被激活。

芒草是亚洲热带地区一种著名的草本植物。以往的研究报道了核桃的抗癌活性，但其对宫颈癌的分子机制尚不完全清楚。因此，我们的研究旨在探讨其对HeLa系的影响，并分析分子相互作用。首先，对RNA-Seq reads进行处理，进行差异表达分析。采用独创通路分析（Ingenuity Pathway Analysis， IPA）方法，鉴定出3个重要基因（CASP9、FOXO4、TNFSF15）的5条细胞死亡相关通路。本研究揭示了子宫癌DCM部分介导细胞死亡机制的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.