Galbanic acid delays the development of seizures by modulating the expression of TNFα, IL1β, and TLR4 genes and reducing hippocampal nitrite levels and may be useful in the treatment of epilepsy

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-03-15 DOI:10.1016/j.neulet.2025.138200

Najmeh Asgharzadeh , Saeid Ghavamnia , Hossein Amini-Khoei , Zahra Lorigooini , Marzieh Mardani , Elham Bijad , Diana shahrani , Mohamad shahrani , Mehrdad Shahrani korrani

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引用次数: 0

Abstract

Introduction and Objective

Epilepsy is one of the most common chronic brain disorders worldwide. This study aimed to determine the effect of Galbanic acid on seizures.

Materials and Methods

In this study, 40 NMRI mice were used and divided into 4 groups. The control group received 10 mg/kg normal saline. Three groups received Galbanic acid at doses of 10, 20, and 40 mg/kg. The positive control group received diazepam with a dose of 10 mg/kg. All injections were made intraperitoneally for one week. Seizures were induced in all mice by injection of 90 mg/kg Pentylenetetrazole (PTZ). Then the latency of seizure onset in each of the experimental groups was recorded. After anesthesia with Ketamine 100 mg/kg and Xylazine 10 mg/kg, blood samples were taken and after decapitation, the hippocampus was isolated and the expression of αTNF-, IL1β, and TLR4 genes in this region was measured.

Results

Galbanic acid increased the delay in seizure onset, decreased brain and serum nitrite levels, and decreased the expression of αTNF-, IL1β, and TLR4 genes in brain tissue compared to the control group (P < 0.01, p < 0.001).

Conclusion

Galbanic acid reduced nitrite and the expression of αTNF-, IL1β, and TLR4, leading to a decrease in neuroinflammation and, as a result, a delay in seizure onset in mice.

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高良姜酸通过调节 TNFα、IL1β 和 TLR4 基因的表达，降低海马亚硝酸盐水平，从而延缓癫痫发作的发展，可能有助于治疗癫痫。

简介和目的：癫痫是世界上最常见的慢性脑部疾病之一。本研究旨在确定Galbanic酸对癫痫发作的影响。材料与方法：本研究选用40只NMRI小鼠，分为4组。对照组给予生理盐水10 mg/kg。三组服用剂量分别为10、20和40 mg/kg的Galbanic酸。阳性对照组给予地西泮10 mg/kg。所有注射均为腹腔注射，持续一周。所有小鼠均注射90 mg/kg戊四唑（PTZ）诱导癫痫发作。然后记录各组癫痫发作潜伏期。氯胺酮100 mg/kg、噻嗪10 mg/kg麻醉后采血，断头后分离海马，测定该区域αTNF-、il - 1β、TLR4基因的表达。结果：与对照组相比，Galbanic酸增加了癫痫发作的延迟时间，降低了脑和血清亚硝酸盐水平，降低了脑组织中αTNF-、il - 1β和TLR4基因的表达(P 结论：Galbanic酸减少了亚硝酸盐和αTNF-、il - 1β和TLR4的表达，导致神经炎症减少，从而延迟了小鼠的癫痫发作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

期刊介绍： Neuroscience Letters is devoted to the rapid publication of short, high-quality papers of interest to the broad community of neuroscientists. Only papers which will make a significant addition to the literature in the field will be published. Papers in all areas of neuroscience - molecular, cellular, developmental, systems, behavioral and cognitive, as well as computational - will be considered for publication. Submission of laboratory investigations that shed light on disease mechanisms is encouraged. Special Issues, edited by Guest Editors to cover new and rapidly-moving areas, will include invited mini-reviews. Occasional mini-reviews in especially timely areas will be considered for publication, without invitation, outside of Special Issues; these un-solicited mini-reviews can be submitted without invitation but must be of very high quality. Clinical studies will also be published if they provide new information about organization or actions of the nervous system, or provide new insights into the neurobiology of disease. NSL does not publish case reports.