Recent advancements in aptamers as promising nanotool for therapeutic and diagnostic applications

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-03-14 DOI:10.1016/j.ab.2025.115844

Omar Awad Alsaidan

引用次数: 0

Abstract

Aptamers are single-strand oligonucleotide molecules having certain structural interactions which allow them to bind to specific targets. Modified nucleotides are added during or after a selection procedure like Systematic Evolution of Ligands by Exponential Enrichment i.e., SELEX to enhance the characteristics and functionality of aptamers. Aptamers are extensible molecular tools with several uses such as in drug administration, biosensing, bioimaging, drug therapies and diagnostics. The ability to detect is improved by using aptamer-based sensors in conjunction with biological molecules among other sensing techniques. Chemical modification, and strong resistance to denaturation, aptamers are appropriate biological recognizing agents for developing sensitive and repeatable aptasensors. This review discusses the most current developments in the aptamers, SELEX method, applications of aptamers as innovative diagnostic, therapeutic & theragnostic tool along with major limitations & prospective directions in the future.

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适体作为治疗和诊断应用的纳米工具的最新进展。

适配体是单链寡核苷酸分子，具有一定的结构相互作用，使其能够与特定靶标结合。修饰的核苷酸是在选择过程中或之后添加的，如配体的系统进化指数富集，即SELEX，以增强适配体的特征和功能。适配体是一种可扩展的分子工具，具有多种用途，如药物给药，生物传感，生物成像，药物治疗和诊断。通过将基于适配体的传感器与其他传感技术中的生物分子结合使用，检测能力得到了提高。适体具有化学修饰、抗变性能力强等特点，是开发灵敏、可重复的适体传感器的理想生物识别剂。本文综述了适体的最新发展，SELEX方法，适体作为创新诊断、治疗和诊断工具的应用，以及未来的主要局限性和展望方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.