Formation of Complex Spatial Structures of DNA in the Process of Ab Initio Synthesis

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2025-03-17 DOI:10.1134/S0006350924701069

N. V. Zyrina, O. M. Selivanova, E. V. Shevchenko, V. N. Antipova

引用次数: 0

Abstract

Products with a complex spatial organization were formed during ab initio synthesis using the large fragment of the Bst DNA polymerase. Using the analysis of AFM images of products obtained with the addition of a minimal amount of the nicking endonuclease Nt. BspD6I during this synthesis it was possible to describe the structures that formed as a result of this synthesis and suggest that their formation occurs by the mechanism of recombination-dependent replication.

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从头合成过程中DNA复杂空间结构的形成

在从头合成过程中，利用Bst DNA聚合酶的大片段形成了具有复杂空间组织的产物。在此合成过程中，通过对添加少量缺口内切酶Nt. BspD6I获得的产物的AFM图像进行分析，可以描述合成过程中形成的结构，并表明它们的形成是通过重组依赖复制的机制发生的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.