Antonio Calisi, Mario Angelelli, Davide Gualandris, Davide Rotondo, Giorgio Mancinelli, Francesco Dondero
{"title":"Locomotion (behavioural) test in the terrestrial oligochaetes Eisenia exposed to carbamate model substance.","authors":"Antonio Calisi, Mario Angelelli, Davide Gualandris, Davide Rotondo, Giorgio Mancinelli, Francesco Dondero","doi":"10.1007/s10646-025-02870-3","DOIUrl":null,"url":null,"abstract":"<p><p>This study examines earthworm behaviour by combining locomotion-based motility assessments with evaluations of acetylcholinesterase (AChE) inhibition. Motility analysis revealed significant differences in the two-dimensional movement patterns of earthworms exposed to carbamate pesticides compared to those in the control group, indicating altered trajectories. AChE assays demonstrated a pronounced inhibitory effect on enzyme activity in exposed earthworms relative to unexposed individuals. Both univariate and multivariate analyses confirmed that the pesticide contaminant significantly affects AChE activity as well as the quantitative and directional characteristics of earthworm movement. These results suggest that behavioural testing in earthworms is a valuable tool for understanding the impact of pesticides on non-target organisms and the environment.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":"692-699"},"PeriodicalIF":2.4000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049370/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecotoxicology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s10646-025-02870-3","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/15 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study examines earthworm behaviour by combining locomotion-based motility assessments with evaluations of acetylcholinesterase (AChE) inhibition. Motility analysis revealed significant differences in the two-dimensional movement patterns of earthworms exposed to carbamate pesticides compared to those in the control group, indicating altered trajectories. AChE assays demonstrated a pronounced inhibitory effect on enzyme activity in exposed earthworms relative to unexposed individuals. Both univariate and multivariate analyses confirmed that the pesticide contaminant significantly affects AChE activity as well as the quantitative and directional characteristics of earthworm movement. These results suggest that behavioural testing in earthworms is a valuable tool for understanding the impact of pesticides on non-target organisms and the environment.
期刊介绍：
Ecotoxicology is an international journal devoted to the publication of fundamental research on the effects of toxic chemicals on populations, communities and terrestrial, freshwater and marine ecosystems. It aims to elucidate mechanisms and processes whereby chemicals exert their effects on ecosystems and the impact caused at the population or community level. The journal is not biased with respect to taxon or biome, and papers that indicate possible new approaches to regulation and control of toxic chemicals and those aiding in formulating ways of conserving threatened species are particularly welcome. Studies on individuals should demonstrate linkage to population effects in clear and quantitative ways. Laboratory studies must show a clear linkage to specific field situations. The journal includes not only original research papers but technical notes and review articles, both invited and submitted. A strong, broadly based editorial board ensures as wide an international coverage as possible.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
