Rapid Screening of 60 Types of Natural Toxins in Whole Blood by UPLC-Q/Orbitrap HRMS.

Q3 Medicine

法医学杂志 Pub Date : 2024-12-25 DOI:10.12116/j.issn.1004-5619.2024.340202

Zhu-Tao Guo, Qian-Ya Deng, Si-Yang He, Shui-Qing Zheng, Rong Wang, Wen-Juan Sun, Chen Liang, Chun-Fang Ni

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引用次数: 0

Abstract

Objectives: To establish a rapid screening method for 60 types of natural toxins in whole blood by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry (UPLC-Q/Orbitrap HRMS).

Methods: The chromatographic and mass spectrometric information of 60 standard samples of natural toxins were analyzed and recorded, and a screening database was built. Whole blood was pretreated by protein precipitation method combined with ultrasonic-assisted dispersion, and then a Hypersil GOLD^TM C₁₈ column was used with 5 mmol/L ammonium formate aqueous solution (containing 0.1% formic acid) and acetonitrile as mobile phase for gradient elution. In the positive ion mode, the data were collected in full scan/data-dependent secondary scan (Full MS/dd-MS²) mode. Based on the established screening library, the rapid screening of 60 types of natural toxins in whole blood was realized by TraceFinder software.

Results: A UPLC-Q/Orbitrap HRMS method was developed for the screening of 60 types of natural toxins in whole blood. Except for the limit of detection (LOD) of oxymatrine (20 ng/mL) and strophanthidin (40 ng/mL), the LOD for the other 58 natural toxins was in the range of 0.05-5 ng/mL.

Conclusions: This method has a simple and efficient pretreatment process and can achieve rapid screening of 60 types of natural toxins in whole blood.

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UPLC-Q/Orbitrap HRMS快速筛选全血中60种天然毒素

目的：建立超高效液相色谱-四极杆/静电场轨道阱高分辨质谱（UPLC-Q/ orbitrap HRMS）快速筛选全血中60种天然毒素的方法。方法：对60份天然毒素标准品的色谱和质谱信息进行分析和记录，建立筛选数据库。全血采用蛋白质沉淀法联合超声辅助分散预处理，以5 mmol/L甲酸铵水溶液（含0.1%甲酸）、乙腈为流动相，采用Hypersil GOLDTM C18色谱柱进行梯度洗脱。在正离子模式下，数据采集采用全扫描/数据依赖二次扫描（full MS/dd-MS2）模式。在建立筛选文库的基础上，利用TraceFinder软件实现了对全血中60种天然毒素的快速筛选。结果：建立了全血中60种天然毒素的UPLC-Q/Orbitrap HRMS筛选方法。除氧化苦参碱（20 ng/mL）和桔梗素（40 ng/mL）的检出限外，其余58种天然毒素的检出限均在0.05 ~ 5 ng/mL范围内。结论：该方法预处理工艺简单高效，可实现全血中60种天然毒素的快速筛选。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

法医学杂志 Medicine-Pathology and Forensic Medicine

CiteScore

1.50

自引率

0.00%

发文量

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