In vitro Evaluation of Cytotoxicity, Biocompatibility, and Osteogenic Effect of Two Calcium Silicate Sealers Compared with AH Plus Jet.

Q1 Dentistry

European Journal of Dentistry Pub Date : 2025-03-12 DOI:10.1055/s-0044-1789602

Monique Aparecida de Lima Rios Pitzschk, Carlos Eduardo da Silveira Bueno, Carlos Eduardo Fontana, Alexandre Sigrist De Martin, Daniel Guimarães Pedro Rocha, Carolina Pessoa Stringheta, Ana Grasiela da Silva Limoeiro, Virgilio Marcio Bastos Braga, Caroliny Chavier Guimaraes, Marilia Fagury Videira Marceliano-Alves, Wayne Martins Nascimento, Thiago Sena Guimarães, Rina Andrea Pelegrine

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引用次数: 0

Abstract

Objective: The purpose of the study was to investigate the cytotoxicity, biocompatibility, and osteogenic effect of EndoSequence BCSealer HiFlow (BCH) and Bio-C Sealer (BCS) in osteosarcoma cells SAOS-2 compared with AH Plus Jet.

Materials and methods: For cytotoxicity analysis, the [3-(4,5-dimethyl-thiazole)-2,5-diphenyltetrazolium bromide-MTT; Sigma/Aldrich] method was used after 24, 48, and 72 hours. For cellular bioactivity, alkaline phosphatase enzyme (ALP) was evaluated after 7 and 14 days.

Statistical analysis: MTT assay was assessed using analysis of variance (ANOVA) and Tukey's test and over time using ANOVA and Sidak's test. For ALP analysis, the one-way ANOVA and Student's paired t-tests were used.

Results: BCS was like AH Plus Jet. After 72 hours, BCH and BCS did not differ from AH Plus Jet and showed similar behavior in terms of viability. ALP showed a difference only after 14 days. BCH had the lowest bioactivity value. AH Plus Jet and BCS were like each other.

Conclusion: AHP was more viable for SAOS-2, and the biocompatibility of Calcium silicate-based sealers was acceptable. The present finding assessed the biocompatibility of Calcium silicate bioceramic sealers, such as BCS, EndoSequence BCH, and AH Plus Jet, is acceptable and they have osteogenic effect and bioactivity.

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来源期刊

European Journal of Dentistry Dentistry-Dentistry (all)

CiteScore

5.10

自引率

0.00%

发文量

161

期刊介绍： The European Journal of Dentistry is the official journal of the Dental Investigations Society, based in Turkey. It is a double-blinded peer-reviewed, Open Access, multi-disciplinary international journal addressing various aspects of dentistry. The journal''s board consists of eminent investigators in dentistry from across the globe and presents an ideal international composition. The journal encourages its authors to submit original investigations, reviews, and reports addressing various divisions of dentistry including oral pathology, prosthodontics, endodontics, orthodontics etc. It is available both online and in print.