Genetic characterization of extensively drug-resistant blaCTX-M-27 Shigella sonnei clusters among men who have sex with men in a region of northern Spain

IF 4.9 2区医学 Q1 INFECTIOUS DISEASES

International Journal of Antimicrobial Agents Pub Date : 2025-03-08 DOI:10.1016/j.ijantimicag.2025.107490

Domingo Fernández Vecilla , Mikel Joseba Urrutikoetxea Gutiérrez , María Carmen Nieto Toboso , Kristina Zugazaga Inchaurza , Estíbaliz Ugalde Zárraga , Beatriz Ruiz Estévez , José Luis Díaz de Tuesta del Arco

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引用次数: 0

Abstract

Objective

The convergence of globalization with increased sexual risk behaviours has significantly facilitated the dissemination of multidrug-resistant and extensively drug-resistant clusters of Shigella spp. among men who have sex with men, particularly Shigella sonnei and Shigella flexneri. A cluster of S. sonnei carrying bla_CTX-M-27 caused a European outbreak in 2020–2021, with more than 30 cases in Spain, including two in our institution. In this study, we conducted a retrospective study from October 2022 to December 2023 that included five additional patients with shigellosis caused by a CTX-M-27-producing S. sonnei.

Methods

Genetic characterization was assessed by whole-genome sequencing using the MinION Mk1C device (Oxford Nanopore Technologies, Oxford, UK).

Results

All the isolates presented IncB/O/K/Z or IncFII plasmids, which carried genes conferring resistance to second- and third-generation cephalosporins, cotrimoxazole, azithromycin and quinolones. SNP analysis revealed that neither the strains within this study nor the UK cluster were related to each other.

Conclusions

Different community clusters of extensively drug-resistant S. sonnei strains harbouring bla_CTX-M-27 are spreading in our area, mainly associated with sexual transmission among men who have sex with men.

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来源期刊

International Journal of Antimicrobial Agents 医学-传染病学

CiteScore

21.60

自引率

0.90%

发文量

176

审稿时长

36 days

期刊介绍： The International Journal of Antimicrobial Agents is a peer-reviewed publication offering comprehensive and current reference information on the physical, pharmacological, in vitro, and clinical properties of individual antimicrobial agents, covering antiviral, antiparasitic, antibacterial, and antifungal agents. The journal not only communicates new trends and developments through authoritative review articles but also addresses the critical issue of antimicrobial resistance, both in hospital and community settings. Published content includes solicited reviews by leading experts and high-quality original research papers in the specified fields.