Inconsistencies in the published rabbit ribosomal rRNAs: a proposal for uniformity in sequence and site numbering.

IF 4.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

RNA Pub Date : 2025-05-16 DOI:10.1261/rna.080294.124

Swastik De, Michelle Zhou, Zuben P Brown, Raymond N Burton-Smith, Yaser Hashem, Tatyana V Pestova, Christopher U T Hellen, Joachim Frank

引用次数: 0

Abstract

Examination of all publicly available Oryctolagus cuniculus (rabbit) ribosome cryo-EM structures reveals numerous confusing inconsistencies. First, there are a plethora of single-nucleotide differences among the various rabbit 28S and 18S rRNA structures. Second, two nucleotides are absent from the NCBI Reference Sequence for the 18S rRNA gene. Moving forward, we propose using the Broad Institute's rabbit whole-genome shotgun sequence and numbering to reduce modeling ambiguity and improve consistency between ribosome models.

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已发表的兔核糖体rRNAs的不一致性：序列和位点编号一致性的建议。

检查所有公开可用的兔核糖体冷冻电镜结构揭示了许多令人困惑的不一致。首先，在不同的兔28S和18S rRNA结构中存在过多的单核苷酸差异。其次，18S rRNA基因的NCBI参考序列中缺少两个核苷酸。展望未来，我们建议使用Broad研究所的兔子全基因组霰弹枪序列和编号来减少建模歧义并提高核糖体模型之间的一致性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

RNA 生物-生化与分子生物学

CiteScore

8.30

自引率

2.20%

发文量

101

审稿时长

2.6 months

期刊介绍： RNA is a monthly journal which provides rapid publication of significant original research in all areas of RNA structure and function in eukaryotic, prokaryotic, and viral systems. It covers a broad range of subjects in RNA research, including: structural analysis by biochemical or biophysical means; mRNA structure, function and biogenesis; alternative processing: cis-acting elements and trans-acting factors; ribosome structure and function; translational control; RNA catalysis; tRNA structure, function, biogenesis and identity; RNA editing; rRNA structure, function and biogenesis; RNA transport and localization; regulatory RNAs; large and small RNP structure, function and biogenesis; viral RNA metabolism; RNA stability and turnover; in vitro evolution; and RNA chemistry.