Metabolic engineering of Lactobacilli spp. for disease treatment.

IF 4.3 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbial Cell Factories Pub Date : 2025-03-06 DOI:10.1186/s12934-025-02682-4

Yunpeng Yang, Peijun Yu, Yufei Huang, Wanying Zhang, Yanhong Nie, Changshan Gao

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引用次数: 0

Abstract

Background: A variety of probiotics have been utilized as chassis strains and engineered to develop the synthetic probiotics for disease treatment. Among these probiotics, Lactobacilli, which are generally viewed as safe and capable of colonizing the gastrointestinal tract effectively, are widely used. We review recent advancements in the engineering of Lactobacilli for disease treatment. Specifically, the Lactobacilli that are used for the construction of synthetic probiotics, the application of these engineered strains for diseases treatment, and the therapeutic outcomes of these engineered microbes are summarized in this review. Moreover, the applications of these engineered strains for disease treatment are categorized based on their engineering strategies. Of note, we compare the advantages and disadvantages of various engineering strategies and offer insights for the future development of genetically modified Lactobacillus strains with stable and safe properties.

Short conclusion: Our study comprehensively reviews researches on engineering diverse Lactobacillus strains for disease treatment, categorized by their engineering strategies, and emphasizes the importance of developing synthetic probiotics with stable and safe characteristics to enhance their therapeutic applications.

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来源期刊

Microbial Cell Factories 工程技术-生物工程与应用微生物

CiteScore

9.30

自引率

4.70%

发文量

235

审稿时长

2.3 months

期刊介绍： Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems