Inhibitory effect and mechanism of purified saponin from Polygonatum cyrtonema Hua on human ovarian cancer cell A2780

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology Pub Date : 2025-03-04 DOI:10.1016/j.fct.2025.115368

Qile Xia , Beiqiong Hao , Chenxing Liu, Yan Cao

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引用次数: 0

Abstract

Polygonatum cyrtonema Hua (PcH) is a traditional medicinal and edible homologous plant. Here, the anticancer activity of different purified PcH saponins (PcHs) on the ovarian cancer (OC) cell A2780 were investigated. The PcHs were purified by AB-8. Their effects on the apoptosis, migration and invasion of A2780 were explored by CCK8, wound healing assay, Hoechst 33258 staining, Annexin V-FITC/PI and intracellular reactive oxygen species (ROS) measurement. Moreover, western blot and realtime-PCR confirmation were performed for the associated protein and mRNA targets. The results showed that the content of PcHs increased from 0.31 ± 0.02 mg/mL to 0.79 ± 0.02 mg/mL after purification. The inhibition rate of purified PcHs on A2780 could reach 66.76 ± 0.56%. Specifically, it promoted cell apoptosis, disturbed the cell cycle, increased the ROS levels, regulated the expression levels of pro-apoptotic and anti-apoptotic proteins. PcHs can also regulate the expression level of proteins related to the invasion and migration characteristics of A2780, the mRNA expression level related to the P13K-Akt signal transduction process, and the extracellular matrix-receptor interaction pathway, as well as drug resistance. Overall, this study provides a new idea for the treatment of OC.

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黄精皂苷对人卵巢癌细胞A2780的抑制作用及其机制。

黄精（Polygonatum cyrtonema Hua）是一种传统的药用和食用同源植物。本实验研究了不同纯化的PcH皂苷（PcHs）对卵巢癌（OC）细胞A2780的抗癌作用。用AB-8纯化PcHs。通过CCK8、创面愈合实验、Hoechst 33258染色、Annexin V-FITC/PI和细胞内活性氧（ROS）测定，探讨其对A2780细胞凋亡、迁移和侵袭的影响。此外，对相关蛋白和mRNA靶点进行western blot和real - time- pcr确认。结果表明，纯化后的PcHs含量由0.31±0.02 mg/mL增加到0.79±0.02 mg/mL。纯化后的PcHs对A2780的抑制率可达66.76±0.56%。具体来说，它促进细胞凋亡，扰乱细胞周期，增加ROS水平，调节促凋亡和抗凋亡蛋白的表达水平。PcHs还可以调控A2780侵袭迁移特性相关蛋白的表达水平、P13K-Akt信号转导过程相关mRNA的表达水平、细胞外基质-受体相互作用途径相关mRNA的表达水平以及耐药性。总的来说，本研究为OC的治疗提供了一个新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.