Bastien L'Hermitte, Thomas Chauvet, Sylvie Georgeault-Daguenet, Nicolas Le Yondre, Philippe Jehan, Reynald Gillet, Christine Baysse
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引用次数: 0
Abstract
We developed two Pseudomonas aeruginosa biosensors to detect trans-translation inhibitors in this medically relevant pathogen. These biosensors leverage the red fluorescence produced by the accumulation of protoporphyrin IX, the substrate of ferrochelatase. The first biosensor monitors tmRNA-SmpB-mediated tagging and degradation of ferrochelatase, while the second serves as a control by tracking ClpP1-mediated proteolysis and porphyrin biosynthesis. Both biosensors were tested in wild-type and mutant backgrounds, and red fluorescence was measured relative to absorbance at 600 nm in microtiter plates. The results confirmed a link between fluorescence and trans-translation or proteolysis activity. These biosensors offer a promising tool for high-throughput screening of trans-translation inhibitors in P. aeruginosa.
期刊介绍：
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.
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