Rates of increase and decrease for each block of poly(3-hydroxybutyrate)(P3HB)-b-poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(P3HBV) and P3HBV-b-P3HB during biosynthesis by Ralstonia Eutropha

IF 2.3 4区化学 Q3 POLYMER SCIENCE

Polymer Journal Pub Date : 2024-11-22 DOI:10.1038/s41428-024-00989-4

Takahiko Nakaoki, Haruto Nakamura, Ren Yashita

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Abstract

The number-average molecular weights (Mns) of the first and second blocks of the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV)-b-poly(3-hydroxybutyrate) (P3HB) and P3HB-b-P3HBV block copolymers biosynthesized from Ralstonia eutropha were investigated. Pentanoic acid and glucose were used as carbon sources for the biosynthesis of the blocks of P3HBV and P3HB, respectively. The cultivation time for the first carbon source was fixed at 72 h, and that for the second carbon source was varied. The first and second blocks for P3HBV-b-P3HB and P3HB-b-P3HBV showed a decrease and increase in the Mn, respectively; this indicates that degradation and polymerization proceeded at the initial end of the first block and at the other end of the second block, respectively. In the biosynthesis of P3HBV-b-P3HB, the rates of decrease and increase in the Mn for the P3HBV and P3HB blocks were 9.0 × 103 and 2.9 × 104 g/(mol∙h), respectively. For P3HB-b-P3HBV, the rates of decrease and increase in the Mn were 2.6 × 103 and 2.0 × 103 g/(mol∙h) for P3HB and P3HBV, respectively. The number-average molecular weights (Mns) of the first and second blocks of the poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P3HBV)-b-poly(3-hydroxybutyrate) (P3HB) and P3HB-b-P3HBV block copolymers biosynthesized from Ralstonia eutropha were investigated. The first and second blocks for both P3HBV-b-P3HB and P3HB-b-P3HBV showed a decrease and increase in the Mn, respectively; this indicates that degradation and polymerization proceeded at the initial end of the first block and at the other end of the second block, respectively.

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聚（3-羟基丁酸酯）(P3HB)-b-聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（P3HBV）和P3HBV-b-P3HB在Ralstonia Eutropha生物合成过程中的增减率

研究了以真菌菌为原料合成的聚（3-羟基丁酸酯-co-3-羟基戊酸酯）(P3HBV)-b-聚（3-羟基丁酸酯）（P3HB）和P3HB-b-P3HBV嵌段共聚物的第一段和第二段的数平均分子量（Mns）。戊酸和葡萄糖分别作为碳源用于P3HBV和P3HB的生物合成。第一碳源的培养时间固定为72 h，第二碳源的培养时间变化。P3HBV-b-P3HB和P3HB-b-P3HBV的第一和第二阻滞分别显示Mn降低和升高；这表明降解和聚合分别发生在第一个链段的起始端和第二个链段的另一端。在P3HBV-b-P3HB的生物合成过程中，P3HBV和P3HB片段的Mn降低率为9.0 × 103 g/(mol∙h)，升高率为2.9 × 104 g/(mol∙h)。对于P3HB-b-P3HBV， P3HB和P3HBV的Mn降低率分别为2.6 × 103 g/(mol∙h)和2.0 × 103 g/(mol∙h)。研究了以真菌菌为原料合成的聚（3-羟基丁酸酯-co-3-羟基戊酸酯）(P3HBV)-b-聚（3-羟基丁酸酯）（P3HB）和P3HB-b-P3HBV嵌段共聚物的第一段和第二段的数平均分子量（Mns）。P3HBV-b-P3HB和P3HB-b-P3HBV的第一和第二阻滞分别显示Mn降低和升高；这表明降解和聚合分别发生在第一个链段的起始端和第二个链段的另一端。

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来源期刊

Polymer Journal 化学-高分子科学

CiteScore

5.60

自引率

7.10%

发文量

131

审稿时长

2.5 months

期刊介绍： Polymer Journal promotes research from all aspects of polymer science from anywhere in the world and aims to provide an integrated platform for scientific communication that assists the advancement of polymer science and related fields. The journal publishes Original Articles, Notes, Short Communications and Reviews. Subject areas and topics of particular interest within the journal''s scope include, but are not limited to, those listed below: Polymer synthesis and reactions Polymer structures Physical properties of polymers Polymer surface and interfaces Functional polymers Supramolecular polymers Self-assembled materials Biopolymers and bio-related polymer materials Polymer engineering.