Trypsinized suction blister.

Abstract

Suction blister epidermal grafting (SBEG) is a standard technique for treating vitiligo on challenging areas like fingertips and lips but often results in pigment halos and difficulties with small, curved surfaces. We propose a modified SBEG technique, utilizing trypsinization of one blister graft to improve melanocyte spread and reduce peri-graft halo. Trypsin ethylenediaminetetraacetic acid (EDTA) solution is introduced into a selected blister, creating a cell suspension applied to dermabraded gaps and peripheries, including eyelid margins. At 5-month follow-up, complete re-pigmentation with no halo effect was achieved, indicating this modified approach's potential in enhancing SBEG outcomes for complex sites.