Thi Minh Chau Le, Thi Hanh Dung Tran, Van Phuc Pham, Thanh Long Dang, Khue Tu Duong, Thanh Tan Hua, Thi Ngoc Tuyen Huynh, Thi Hong Nhung Nguyen, Quang Thanh Le, Ba My Nhi Nguyen, Minh Tuan Vo, Phuc Thinh Ong, Nguyen The Nguyen Pham, Christiani Andrade Amorim
{"title":"The first comparative study on the effectiveness of slow freezing and vitrification of human ovarian tissue by xenotransplantation model in Vietnam.","authors":"Thi Minh Chau Le, Thi Hanh Dung Tran, Van Phuc Pham, Thanh Long Dang, Khue Tu Duong, Thanh Tan Hua, Thi Ngoc Tuyen Huynh, Thi Hong Nhung Nguyen, Quang Thanh Le, Ba My Nhi Nguyen, Minh Tuan Vo, Phuc Thinh Ong, Nguyen The Nguyen Pham, Christiani Andrade Amorim","doi":"10.1007/s10815-025-03439-z","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To compare the effectiveness of slow freezing (SF) and vitrification (VT) for ovarian tissue cryopreservation using a xenograft model.</p><p><strong>Methods: </strong>From September 2020 to August 2023, ovarian tissues from patients aged 18 to 37 undergoing benign ovarian surgery were divided into three groups. Group 1: fresh tissues (FC) were immediately fixed for analysis. Group 2 (SF/VT): tissues were cryopreserved by SF or VT without transplantation. Group 3 (SF-T/VT-T): tissues were cryopreserved using SF or VT, followed by transplantation into NOD-SCID mice for one week, after which the tissues were collected and analyzed.</p><p><strong>Results: </strong>A total of 49 ovarian tissue fragments were analyzed. Regardless of the cryopreservation technique, follicle survival, development, function, and vascularization were significantly reduced compared to the FC group, particularly after transplantation (p < 0.001). The survival rate of follicles in the SF-T group was notably higher (90.9%) than in the VT-T group (82.6%) (p < 0.001). For cell proliferation, indicated by Ki-67 positivity, the SF-T group showed a higher median number of positive follicles (2.5; range: 0-18) compared to the VT-T group (2; range: 0-11) (p = 0.04). Neo-vascularization, assessed via CD31 positivity, was significantly greater in the SF-T group (61%) than in the VT-T group (47%) (p = 0.016). Additionally, the median number of AMH-positive follicles was significantly higher in the SF-T group (3; range: 0-23) compared to the VT-T group (2; range: 0-25) (p = 0.03).</p><p><strong>Conclusion: </strong>Both SF and VT are feasible methods for ovarian tissue cryopreservation. SF may be the preferred cryopreservation technique for fertility preservation in cases tissue transplantation is anticipated.</p>","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Assisted Reproduction and Genetics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10815-025-03439-z","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: To compare the effectiveness of slow freezing (SF) and vitrification (VT) for ovarian tissue cryopreservation using a xenograft model.
Methods: From September 2020 to August 2023, ovarian tissues from patients aged 18 to 37 undergoing benign ovarian surgery were divided into three groups. Group 1: fresh tissues (FC) were immediately fixed for analysis. Group 2 (SF/VT): tissues were cryopreserved by SF or VT without transplantation. Group 3 (SF-T/VT-T): tissues were cryopreserved using SF or VT, followed by transplantation into NOD-SCID mice for one week, after which the tissues were collected and analyzed.
Results: A total of 49 ovarian tissue fragments were analyzed. Regardless of the cryopreservation technique, follicle survival, development, function, and vascularization were significantly reduced compared to the FC group, particularly after transplantation (p < 0.001). The survival rate of follicles in the SF-T group was notably higher (90.9%) than in the VT-T group (82.6%) (p < 0.001). For cell proliferation, indicated by Ki-67 positivity, the SF-T group showed a higher median number of positive follicles (2.5; range: 0-18) compared to the VT-T group (2; range: 0-11) (p = 0.04). Neo-vascularization, assessed via CD31 positivity, was significantly greater in the SF-T group (61%) than in the VT-T group (47%) (p = 0.016). Additionally, the median number of AMH-positive follicles was significantly higher in the SF-T group (3; range: 0-23) compared to the VT-T group (2; range: 0-25) (p = 0.03).
Conclusion: Both SF and VT are feasible methods for ovarian tissue cryopreservation. SF may be the preferred cryopreservation technique for fertility preservation in cases tissue transplantation is anticipated.
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
