Exploration of shrimp and their environments for the detection of antibiotic resistance genes of Vibrio parahaemolyticus and spectrophotometry of shrimp muscles for heavy metals and their human health risk assessment in Bangladesh.

IF 2.1 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2025-02-28 DOI:10.1016/j.jfp.2025.100475

M Sohidullah, Md Hamidur Rahman, Abu Sayeed, Sadia Rahman, Linta Yesmin, Md Imran Chowdhury, Md Jannat Hossain, Muhammad Ashiqul Alam, Md Salauddin, Md Habibur Rahman, Md Tazinur Rahman, Sayeed Khaled Sabbir

{"title":"Exploration of shrimp and their environments for the detection of antibiotic resistance genes of Vibrio parahaemolyticus and spectrophotometry of shrimp muscles for heavy metals and their human health risk assessment in Bangladesh.","authors":"M Sohidullah, Md Hamidur Rahman, Abu Sayeed, Sadia Rahman, Linta Yesmin, Md Imran Chowdhury, Md Jannat Hossain, Muhammad Ashiqul Alam, Md Salauddin, Md Habibur Rahman, Md Tazinur Rahman, Sayeed Khaled Sabbir","doi":"10.1016/j.jfp.2025.100475","DOIUrl":null,"url":null,"abstract":"Through deteriorating the quality of shrimp, Vibrio parahaemolyticus and heavy metals have become threatened to food safety. The study was conducted to explore shrimp and their environments for antibiotic resistance genes of V. parahaemolyticus and perform spectrophotometry of shrimp muscles for heavy metals and their human health risk assessment. In total, 130 samples (shrimp, water, and sediment) were aseptically collected from 27 ponds in four areas of Khulna and Satkhira districts where the number of water and sediments were corresponded to the number of ponds and the number of shrimps differed from pond to pond. V. parahaemolyticus were detected by cultural, staining, biochemical, and molecular techniques targeting groEL, tetA, tetB, tetC, and blaTEM genes. Disc diffusion assay and bivariate analysis were performed for investigating antibiotic resistance profiles of V. parahaemolyticus. Cadmium, chromium, lead, zinc, and iron were measured by AAS (atomic absorption spectrometry) in shrimp. Among 39 isolates (23 from shrimp, 7 from water, 9 from sediment), real-time PCR (polymerase chain reaction) detected 20 of 27 as positive for groEL, 12 of 20 for tetA, 13 for tetB, 12 for tetC, and 1 for blaTEM. V. parahaemolyticus were highly resistant to tetracycline and ampicillin. Bivariate analysis revealed a significant correlation between the antibiotics. 51.28% isolates were MDR (multidrug resistant), and the MAR (multiple antibiotic resistance) indices ranged from 0.08-0.6. The highest average concentration for Cd was in Debhata, Pb in Dumuria, Cr in Kaliganj, Zn and Fe in Satkhira Sadar. THQ (target hazard quotients) of >1 for Fe in all sampling sites showed higher level of HI (hazard index). No determined TR (target cancer risk) value exceeded the recommended value (<10-4). The study emphasizes the significance of adopting extensive surveillance and monitoring of a large number of shrimp farms for effective antibiotic management and sustainable shrimp production.","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100475"},"PeriodicalIF":2.1000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of food protection","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.jfp.2025.100475","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Through deteriorating the quality of shrimp, Vibrio parahaemolyticus and heavy metals have become threatened to food safety. The study was conducted to explore shrimp and their environments for antibiotic resistance genes of V. parahaemolyticus and perform spectrophotometry of shrimp muscles for heavy metals and their human health risk assessment. In total, 130 samples (shrimp, water, and sediment) were aseptically collected from 27 ponds in four areas of Khulna and Satkhira districts where the number of water and sediments were corresponded to the number of ponds and the number of shrimps differed from pond to pond. V. parahaemolyticus were detected by cultural, staining, biochemical, and molecular techniques targeting groEL, tetA, tetB, tetC, and bla_TEM genes. Disc diffusion assay and bivariate analysis were performed for investigating antibiotic resistance profiles of V. parahaemolyticus. Cadmium, chromium, lead, zinc, and iron were measured by AAS (atomic absorption spectrometry) in shrimp. Among 39 isolates (23 from shrimp, 7 from water, 9 from sediment), real-time PCR (polymerase chain reaction) detected 20 of 27 as positive for groEL, 12 of 20 for tetA, 13 for tetB, 12 for tetC, and 1 for bla_TEM. V. parahaemolyticus were highly resistant to tetracycline and ampicillin. Bivariate analysis revealed a significant correlation between the antibiotics. 51.28% isolates were MDR (multidrug resistant), and the MAR (multiple antibiotic resistance) indices ranged from 0.08-0.6. The highest average concentration for Cd was in Debhata, Pb in Dumuria, Cr in Kaliganj, Zn and Fe in Satkhira Sadar. THQ (target hazard quotients) of >1 for Fe in all sampling sites showed higher level of HI (hazard index). No determined TR (target cancer risk) value exceeded the recommended value (<10^-4). The study emphasizes the significance of adopting extensive surveillance and monitoring of a large number of shrimp farms for effective antibiotic management and sustainable shrimp production.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.