[The Significance of miR-122 in Early Screening of Hepatitis B Infection in Blood Donors].

Q4 Medicine

中国实验血液学杂志 Pub Date : 2025-02-01 DOI:10.19746/j.cnki.issn.1009-2137.2025.01.033

You-Zhi Zhan, Wei-Mei Jiang, Fang Chen, Shou Lin, Hong-Keng Lin

{"title":"[The Significance of miR-122 in Early Screening of Hepatitis B Infection in Blood Donors].","authors":"You-Zhi Zhan, Wei-Mei Jiang, Fang Chen, Shou Lin, Hong-Keng Lin","doi":"10.19746/j.cnki.issn.1009-2137.2025.01.033","DOIUrl":null,"url":null,"abstract":"Objective: To study the correlation between miR-122 and early HBV infection and analyze its application value in early infection of voluntary blood donors.Methods: A total of 150 samples from voluntary blood donors in Fujian Blood Center from May 2021 to July 2022 were collected and divided into group N (normal group), group E (ELISA single positive group), and group D (both ELISA and nucleic acid positive group), and the general information of the three groups of blood donors was collected. Total RNA was extracted from the three groups of samples, and the expression level of miR-122 was detected by qRT-PCR. The expression differences of miR-122 among the three groups of samples were statistically analyzed, and the correlation between the expression level of miR-122 in group D and its HBV DNA copy number was analyzed. The miRNA database was used to predict the potential target genes of miRNA and perform bioinformatics analysis.Results: There was no statistical difference in gender, education level, and occupation distribution among the three groups, but the age distribution and number of blood donations among different groups were statistically significant. Compared with group N, the relative expression levels of miR-122 in the plasma of group E and group D were significantly downregulated (P < 0.05); the relative expression level of miR-122 in group D was more significantly downregulated than that in group E (P < 0.001). Pearson correlation analysis showed that the expression level of miR-122 in group D was negatively correlated with the HBV-DNA copy number (R 2=-0.804,P < 0.01). Two potential target genes were screened using the miRNA database: ALDOA(aldolase A) and PKM (pyruvate kinase). GO analysis results showed that the potential target genes of miRNA mainly involved in biological processes including cell homeostasis and regulation of transcriptional processes.Conclusion: Downregulation of miR-122 expression is closely related to early HBV infection and replication activity.","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 1","pages":"224-229"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实验血液学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.01.033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To study the correlation between miR-122 and early HBV infection and analyze its application value in early infection of voluntary blood donors.

Methods: A total of 150 samples from voluntary blood donors in Fujian Blood Center from May 2021 to July 2022 were collected and divided into group N (normal group), group E (ELISA single positive group), and group D (both ELISA and nucleic acid positive group), and the general information of the three groups of blood donors was collected. Total RNA was extracted from the three groups of samples, and the expression level of miR-122 was detected by qRT-PCR. The expression differences of miR-122 among the three groups of samples were statistically analyzed, and the correlation between the expression level of miR-122 in group D and its HBV DNA copy number was analyzed. The miRNA database was used to predict the potential target genes of miRNA and perform bioinformatics analysis.

Results: There was no statistical difference in gender, education level, and occupation distribution among the three groups, but the age distribution and number of blood donations among different groups were statistically significant. Compared with group N, the relative expression levels of miR-122 in the plasma of group E and group D were significantly downregulated (P < 0.05); the relative expression level of miR-122 in group D was more significantly downregulated than that in group E (P < 0.001). Pearson correlation analysis showed that the expression level of miR-122 in group D was negatively correlated with the HBV-DNA copy number (R ²=-0.804,P < 0.01). Two potential target genes were screened using the miRNA database: ALDOA(aldolase A) and PKM (pyruvate kinase). GO analysis results showed that the potential target genes of miRNA mainly involved in biological processes including cell homeostasis and regulation of transcriptional processes.

Conclusion: Downregulation of miR-122 expression is closely related to early HBV infection and replication activity.

查看原文本刊更多论文

[miR-122 在献血者乙型肝炎感染早期筛查中的意义】。］

目的：研究miR-122与早期HBV感染的相关性，分析其在无偿献血者早期感染中的应用价值。方法：采集2021年5月至2022年7月福建省血液中心无偿献血者150份血样，分为N组（正常组）、E组（ELISA单阳性组）和D组（ELISA单阳性组和核酸双阳性组），收集三组献血者的一般信息。提取三组样本的总RNA，采用qRT-PCR检测miR-122的表达水平。统计分析三组样本中miR-122的表达差异，分析D组miR-122表达水平与其HBV DNA拷贝数的相关性。利用miRNA数据库预测miRNA潜在靶基因并进行生物信息学分析。结果：三组患者的性别、文化程度、职业分布无统计学差异，但各组患者的年龄分布和献血次数有统计学意义。与N组比较，E组和D组血浆中miR-122的相对表达量均显著下调（P < 0.05）；D组miR-122的相对表达量明显低于E组（P < 0.001）。Pearson相关分析显示，D组miR-122的表达水平与HBV-DNA拷贝数呈负相关（r2 =-0.804,P < 0.01）。使用miRNA数据库筛选两个潜在的靶基因：ALDOA（醛缩酶A）和PKM（丙酮酸激酶）。GO分析结果显示，miRNA的潜在靶基因主要参与细胞稳态和转录调控等生物学过程。结论：miR-122表达下调与HBV早期感染及复制活性密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊