The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats.

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Elham Ghajari, Marziyeh Tavalaee, Mohammad Hassan Meshkibaf, Maurizio Dattilio, Mohammad Hossein Nasr-Esfahani
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引用次数: 0

Abstract

Objective: This study aims to investigate the effects of sodium hydrogen sulphide (NaHS) supplementation, a hydrogen sulphide (H2S) donor, on oxidant and antioxidant markers, as well as sperm function in rats with experimentally induced varicocele.

Materials and methods: In this experimental study, 55 male Wistar rats were assigned to varicocele (n=25), control (n=20), and sham (n=10) groups. In the varicocele group, five rats received NaHS treatment immediately after surgery for four months and ten rats were treated two to four months after surgery. The remaining ten rats in the varicocele group received no treatment. Similar protocols were followed for the control groups. At the end of four months, all rats were sacrificed, and assessments were made for sperm parameters that included function tests, and testicular malondialdehyde (MDA) and total antioxidant capacity (TAC).

Results: Varicocele induction significantly impaired sperm parameters and sperm function tests. NaHS treatment for two months increased sperm concentrations, while treatment for two and four months improved motility, chromatin status, and intracellular reactive oxygen species (ROS) compared to untreated varicocele rats. After four months, NaHS treatment reduced testicular MDA levels. Testicular TAC significantly increased after two months but decreased after four months of treatment in the varicocele group (P<0.05).

Conclusion: NaHS treatment improved sperm parameters and reduced oxidative stress in varicocele rats. The observed effects depended on the treatment duration.

硫化氢钠对精索静脉曲张大鼠精子功能及氧化应激的影响。
目的:研究添加硫化氢(H2S)供体硫化氢钠(NaHS)对实验性精索静脉曲张大鼠氧化、抗氧化指标及精子功能的影响。材料与方法:将55只雄性Wistar大鼠分为精索静脉曲张组(n=25)、对照组(n=20)和假手术组(n=10)。精索静脉曲张组5只术后立即给予NaHS治疗4个月,10只术后2 ~ 4个月给予NaHS治疗。精索静脉曲张组其余10只大鼠不予治疗。对照组也遵循了类似的方案。四个月后,所有大鼠均被处死,并对精子参数进行评估,包括功能测试、睾丸丙二醛(MDA)和总抗氧化能力(TAC)。结果:精索静脉曲张诱导显著损害精子参数和精子功能。与未治疗的精索静脉曲张大鼠相比,NaHS治疗2个月增加了精子浓度,而治疗2个月和4个月改善了运动能力、染色质状态和细胞内活性氧(ROS)。四个月后,NaHS治疗降低了睾丸MDA水平。精索静脉曲张组睾丸TAC在治疗2个月后显著升高,治疗4个月后显著降低(p结论:NaHS治疗可改善精索静脉曲张大鼠精子参数,降低氧化应激。观察到的效果取决于治疗时间。
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来源期刊
Cell Journal
Cell Journal CELL BIOLOGY-
CiteScore
3.40
自引率
5.00%
发文量
0
审稿时长
12 months
期刊介绍: The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.
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