Jonathan B Lin, Hani El Helwe, Henisk Falah, Bruno L Hammerschlag, Stephanie A Schultz, George Baldwin, Yixi Xue, Ryan A Vasan, Christian Song, Kristine Lo, Austin Meeker, Silas L Wang, Pia Kivisäkk, David Solá-Del Valle, Milica A Margeta
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引用次数: 0
Abstract
Purpose: The purpose of this study was to evaluate the relationship between serum and aqueous humor (AH) neurofilament light chain (NfL) and to determine whether serum NfL is elevated in patients undergoing ocular surgery who have glaucoma compared with those who do not.
Methods: In this single-center, case-control study, we enrolled patients with various types and stages of glaucoma undergoing planned ophthalmic surgery as part of their routine care and compared them with patients without glaucoma undergoing phacoemulsification for age-related cataract. We recruited 110 patients with glaucoma and 113 patients without glaucoma and collected AH and blood from these participants. Levels of AH and serum NfL were quantified using the Single-Molecule Array (Simoa) NF-light assay (Quanterix). Clinical information was obtained by reviewing the medical records.
Results: In a model controlling for age and body mass index (BMI), AH NfL was significantly elevated in patients with glaucoma compared with controls (P < 0.001). In contrast, after controlling for age, BMI, and Mini Mental Status Examination (MMSE) scores, serum NfL was not elevated in patients with glaucoma compared with controls (P = 0.81).
Conclusions: Although our findings validate AH NfL as a marker of glaucomatous neurodegeneration, no such evidence was found for serum NfL.
Translational relevance: NfL levels in AH may be a molecular marker of retinal ganglion cell health in glaucoma; in contrast, serum NfL has limited utility for monitoring glaucomatous neurodegeneration.
期刊介绍：
Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO.
The journal covers a broad spectrum of work, including but not limited to:
Applications of stem cell technology for regenerative medicine,
Development of new animal models of human diseases,
Tissue bioengineering,
Chemical engineering to improve virus-based gene delivery,
Nanotechnology for drug delivery,
Design and synthesis of artificial extracellular matrices,
Development of a true microsurgical operating environment,
Refining data analysis algorithms to improve in vivo imaging technology,
Results of Phase 1 clinical trials,
Reverse translational ("bedside to bench") research.
TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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