Antifungal and plant-growth promoting potency of Streptomyces rochei against biotic stress caused by Race 4 Fusarium wilt on banana

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-02-26 DOI:10.1016/j.stress.2025.100779

Periakaruppan Jegan , Saraswathy Sethurathinam , Muthuvel Iyyamperumal , Rajangam Jacob , Angappan Kathithachalam , Jayakanthan Mannu , Soman Padmanabhan , Manimaran Gajendiran

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Abstract

Banana is a staple food for millions of individuals, especially in regions with tropical and subtropical climates. Nevertheless, the cultivation of banana is under significant threat from Fusarium wilt, a harmful biotic stress transmitted through the soil and caused by Fusarium oxysporum f. sp. cubense (Foc). This disease has the potential to devastate and infect almost all the varieties of bananas, especially with the emergence of Foc race 4. This study investigates the possibility of Streptomyces rochei AMBEAROOT2, isolated from the banana, used as a biocontrol agent against Fusarium wilt in banana. The molecular characterization of ten Foc isolates identified Fusarium oxysporum f. sp. cubense TFOC6 as the most virulent and confirmed as race 4. S. rochei AMBEAROOT2 exhibited significant mycelial inhibition (62.3%) of F. oxysporum f. sp. cubense TFOC6 and plant growth-promoting characteristics, including indole-3-acetic acid, biofilm formation, and exopolysaccharide synthesis. Gas Chromatography-Mass Spectroscopy analysis identified metabolites produced by S. rochei AMBEAROOT2, with molecular docking investigations revealed strong binding affinities of compounds viz., 3-phenyl-3-p-tolyl-1-(2,4,6-trimethyl-phenyl)-propane-1-one, 19-acetoxy-4,4-dimethyl-, oxime, Androst-5-en-3-one, and Pyrano [4,3] benzopyran-1,9-dione to key virulence proteins (Catalase-peroxidase, Kynureninase, Penta functional AROM polypeptide, and Ribose phosphate pyrophosphokinase) of Foc. In greenhouse conditions, micro-propagated banana plantlets treated with S. rochei AMBEAROOT2 demonstrated enhanced growth, improved physiological traits, and higher levels of defense enzymes compared to those inoculated with F. oxysporum f. sp. cubense TFOC6 alone. It also induced systemic resistance and a 100% decrease in the incidence of Fusarium wilt. In conclusion, S. rochei AMBEAROOT2 showed potential antifungal activities, promoted plant growth, and could be used to manage wilt disease in banana after field evaluation.

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.