{"title":"Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity.","authors":"Junkai Wu, Xiaomeng Zhang, Xiaoqing Liu, Zunlai Sheng, Jianping Hu, Feiyan Zhang","doi":"10.3389/fpls.2025.1495779","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The <i>Cannabis Sativa</i> L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential.</p><p><strong>Methods: </strong>The extraction parameters for CBD from industrial hemp were optimized using the Box-Behnken design and response surface methodology (RSM). The purification process involved characterizing the penetration and desorption profiles of CBD on HPD-100 resin. The <i>in vitro</i> antibacterial activity was assessed by determining the minimum inhibitory concentration (MIC) against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. Antioxidant properties were evaluated using DPPH and ABTS assays, as well as an iron-reducing ability test.</p><p><strong>Results: </strong>After optimization, the extraction rate of CBD reached 0.26 ± 0.02%. The use of HP-100 resin in the purification process resulted in a significant enrichment of CBD content, which was 4.2 times higher than that of the crude extract, with a recovery rate of 83.13%. The MIC against <i>S. aureus</i> was found to be 5 mg/mL, while no inhibitory effect was observed against <i>E. coli</i>. The IC50 values for the DPPH and ABTS assays were 0.1875 mg/mL and 2.988 mg/mL, respectively, indicating the potent antioxidant capacity of CBD. Additionally, CBD demonstrated a strong iron-reducing ability.</p><p><strong>Conclusion: </strong>These findings contribute to the development of CBD for broader applications in various industries, highlighting its potential as a valuable compound in health and wellness sectors.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1495779"},"PeriodicalIF":4.1000,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11842326/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2025.1495779","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: The Cannabis Sativa L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential.
Methods: The extraction parameters for CBD from industrial hemp were optimized using the Box-Behnken design and response surface methodology (RSM). The purification process involved characterizing the penetration and desorption profiles of CBD on HPD-100 resin. The in vitro antibacterial activity was assessed by determining the minimum inhibitory concentration (MIC) against Staphylococcus aureus and Escherichia coli. Antioxidant properties were evaluated using DPPH and ABTS assays, as well as an iron-reducing ability test.
Results: After optimization, the extraction rate of CBD reached 0.26 ± 0.02%. The use of HP-100 resin in the purification process resulted in a significant enrichment of CBD content, which was 4.2 times higher than that of the crude extract, with a recovery rate of 83.13%. The MIC against S. aureus was found to be 5 mg/mL, while no inhibitory effect was observed against E. coli. The IC50 values for the DPPH and ABTS assays were 0.1875 mg/mL and 2.988 mg/mL, respectively, indicating the potent antioxidant capacity of CBD. Additionally, CBD demonstrated a strong iron-reducing ability.
Conclusion: These findings contribute to the development of CBD for broader applications in various industries, highlighting its potential as a valuable compound in health and wellness sectors.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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