“Repair and Fold” DNA Nanotweezers for Measuring DNA Alkylation Repair Mediated by ALKBH

Abstract

We reported a novel DNA lesion-modified nanotweezer that underwent conformational switch upon ALKBH enzymes-mediated lesion repair. We demonstrated that the DNA lesion-modified nanotweezer enabled specifically and rapidly monitoring ALKBH2 and ALKBH3 activities and screening inhibitors. Important, this strategy allowed evaluating ALKBH2-associated drug resistance in cells.