Exploring sulphated polysaccharides from marine bivalves: Unveiling potent anti-gastric cancer activity through cell cycle arrest and apoptosis.

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2025-02-22 DOI:10.1080/10286020.2025.2468319

Deepakrajasekar Padmanaban, Pavithra Rajkumar, Mahalakshmi Vijayakumar, Vennila Jayaprakash, Niranjni Sekar, Rajasekar Valayapathi, Aarthi Azhagudurai, Arulvasu Chinnasamy

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Abstract

This study investigates the extraction of partially purified sulphated polysaccharides from marine bivalves through enzymatic digestion followed by sequential deproteinization and evaluates their anti-gastric cancer activity. Chemical analysis confirmed high uronic acid and sulfate content, with FT-IR validating characteristic functional groups. Donax variabilis polysaccharide demonstrated significant, dose-dependent anti-proliferative effects against AGS cells. Mechanistic studies revealed morphological changes, ROS generation, LDH release, and mitochondrial membrane disruption, leading to apoptosis. Flow cytometry confirmed G0/G1 phase arrest. These findings suggest that D. variabilis sulphated polysaccharides exhibit potent anti-gastric cancer properties, likely through oxidative stress and mitochondrial dysfunction, warranting further investigation for therapeutic applications.

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.