Exploring sulphated polysaccharides from marine bivalves: Unveiling potent anti-gastric cancer activity through cell cycle arrest and apoptosis

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2025-10-03 DOI:10.1080/10286020.2025.2468319

Deepakrajasekar Padmanaban , Pavithra Rajkumar , Mahalakshmi Vijayakumar , Vennila Jayaprakash , Niranjni Sekar , Rajasekar Valayapathi , Aarthi Azhagudurai , Arulvasu Chinnasamy

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引用次数: 0

Abstract

This study investigates the extraction of partially purified sulphated polysaccharides from marine bivalves through enzymatic digestion followed by sequential deproteinization and evaluates their anti-gastric cancer activity. Chemical analysis confirmed high uronic acid and sulfate content, with FT-IR validating characteristic functional groups. Donax variabilis polysaccharide demonstrated significant, dose-dependent anti-proliferative effects against AGS cells. Mechanistic studies revealed morphological changes, ROS generation, LDH release, and mitochondrial membrane disruption, leading to apoptosis. Flow cytometry confirmed G0/G1 phase arrest. These findings suggest that D. variabilis sulphated polysaccharides exhibit potent anti-gastric cancer properties, likely through oxidative stress and mitochondrial dysfunction, warranting further investigation for therapeutic applications.

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从海洋双壳类动物中探索硫酸多糖：通过细胞周期阻滞和细胞凋亡揭示有效的抗胃癌活性。

本研究研究了从海洋双壳类动物中提取部分纯化的硫酸多糖，并对其抗胃癌活性进行了评价。化学分析证实了高尿酸和硫酸盐含量，并用FT-IR验证了特征官能团。唐芦多糖对AGS细胞具有明显的剂量依赖性的抗增殖作用。机制研究显示形态学改变、ROS生成、LDH释放和线粒体膜破坏导致细胞凋亡。流式细胞术证实G0/G1期阻滞。这些发现表明，变异性紫菀硫酸多糖可能通过氧化应激和线粒体功能障碍表现出强大的抗胃癌特性，值得进一步研究其治疗应用。

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.