Staphylococcus species from bovine milk: Prevalence, antibiogram profile, and carriage of methicillin resistance and virulence genes

IF 3.3 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-02-21 DOI:10.1016/j.micpath.2025.107410

Yared Abate Getahun , Solomon Lulie Abey , Tesfaye Sisay Tessema

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引用次数: 0

Abstract

Staphylococcus species, mainly S. aureus, S. intermedius, and S. hyicus species, are the primary causes of intra-mammary infections in cows and causes human food poisoning infections; hence, this study aimed to assess the prevalence, antibiogram, virulence, and methicillin resistance gene profiles of pathogenic Staphylococcus species from bovine milk samples. A cross-sectional study design was employed to collect milk samples from February to August 2022 from 290 lactating cows in 38 small and medium-scale dairy farms. Isolation of pathogenic Staphylococcus species was performed by plate culturing and biochemical tests and the antimicrobial susceptibility profiles were determined by Kirby-Bauer disc diffusion method. Virulence and methicillin-resistant genes were detected using conventional PCR. Logistic regression and Chi-square test were used for data analysis. The overall Staphylococcus species prevalence was 16.6 % (95 % CI = 12.5–21.3). Of these, 52.1 %, 33.3 %, and 14.6 % were S. aureus, S. intermedius, and S. hyicus species, respectively. Farm management, herd size, parity, milk yield and breed risk factors had a significant association with the occurrence of Staphylococcus species. All the isolates showed 100 % resistance to amoxicillin and ampicillin discs, while 41.7 % (95 % CI = 27.6–56.8) of the isolates showed multidrug resistance. The study revealed that 68.8 %,33/48 (95 % CI = 53.7–81.3) of the pathogenic Staphylococcus isolates carried one or more of the virulence and/or methicillin resistance genes. The mecA, hlb, hla, icaD, pvl, tsst-1, and sec genes were detected in 58.3 %, 54.2 %, 50 %, 41.7 %, 29.2 %, 10.4 %, and 4.2 %, respectively, from 48 Staphylococcus isolates. The study revealed that pathogenic Staphylococcus isolates carried various virulence and became multidrug resistant; which necessitates employing Staphylococcus caused mastitis control and prevention measures.

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)