Profile of circular RNAs in bovine mammary tissues infected with Staphylococcus aureus

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-02-21 DOI:10.1007/s00203-025-04269-4

Li Zhou, Zhuoma Luoreng, Xingping Wang

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Abstract

Mastitis is one of the most common diseases in cattle. The causes are complex, and the disease tends to recur and its difficult to cure, resulting in significant economic losses to the global dairy industry each year. Circular RNAs (circRNAs), a class of endogenous non-coding RNAs (ncRNAs), have recently been recognized as key regulators in inflammatory diseases. However, little is known about the roles of circRNAs in mastitis caused by Staphylococcus aureus (S. aureus) infection. In this study, bovine mammary gland tissue was treated with S. aureus (10⁵ CFU/ml, M_S) and PBS (control, M_C) and RNA-sequencing was used to explore the effect of S. aureus on circRNA expression in the tissue. The results showed that compared with the M_C group, 202 differentially expressed circRNAs (DE circRNAs) were identified in the M_S group, including 105 up-regulated and 97 down-regulated circRNAs. Gene Ontology (GO) analysis showed that most of the DE circRNAs were enriched in categories such as transcription by RNA polymerase II, transcription factor complexes, and oxidoreductase activity, while Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed significant enrichment in pathways involved in thyroid hormone, FoxO, and cell cycle functions. Seven circRNAs associated with bovine mastitis were also identified (novel_circ_0016953, novel_circ_0001266, novel_circ_0015099, novel_circ_0008169, novel_circ_0001807, novel_circ_0016220, and novel_circ_0009731). The results of this study lay a theoretical foundation for further investigation into the mechanism underlying bovine mastitis and provide valuable references for subsequent research on bovine mastitis.

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感染金黄色葡萄球菌的牛乳腺组织环状rna谱

乳腺炎是牛最常见的疾病之一。其病因复杂，易复发且难以治愈，每年给全球乳制品行业造成重大经济损失。环状rna （circRNAs）是一类内源性非编码rna (ncRNAs)，最近被认为是炎症性疾病的关键调节因子。然而，对于环状rna在金黄色葡萄球菌（S. aureus）感染引起的乳腺炎中的作用知之甚少。本研究用金黄色葡萄球菌（105 CFU/ml, M_S）和PBS（对照，M_C）处理牛乳腺组织，采用rna测序方法探讨金黄色葡萄球菌对组织中circRNA表达的影响。结果显示，与M_C组相比，M_S组共鉴定出202个差异表达circRNAs (DE circRNAs)，其中105个表达上调，97个表达下调。基因本体（GO）分析显示，大多数DE环状RNA在RNA聚合酶II、转录因子复合物和氧化还原酶活性等转录类别中富集，而京都基因与基因组百科全书（KEGG）分析显示，在甲状腺激素、FoxO和细胞周期功能相关的途径中富集。还鉴定了7个与牛乳腺炎相关的circrna （novel_circ_0016953、novel_circ_0001266、novel_circ_0015099、novel_circ_0008169、novel_circ_0001807、novel_circ_0016220和novel_circ_0009731）。本研究结果为进一步探讨牛乳腺炎发病机制奠定了理论基础，并为后续牛乳腺炎的研究提供了有价值的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.