Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH).

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Reports Pub Date : 2025-02-20 DOI:10.1007/s11033-025-10277-w

M Cecilia Giardini, Fabián H Milla, Claudia A Conte, Silvia B Lanzavecchia, Mariela Nieves

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引用次数: 0

Abstract

Background: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X₁, X₂) and two Y (Y₅, Y₆) variants. Our aim was to explore the molecular composition of the sex chromosomes identified in the laboratory strain Af-Y-short due to it possesses the most frequent sex chromosomes variants, and the smallest Y chromosome found in wild populations and laboratory colonies of A. fraterculus sp. 1.

Methods and results: Whole Comparative Genomic Hybridization (W-CGH) was applied to the Af-Y-short laboratory strain to identify potential differences in the size and composition of highly repetitive DNA blocks between individuals, morphs or sexes. Our results showed that the X₁ and X₂ share complete homology in euchromatic regions, also sharing similar sequences with the Y₅ chromosome in the centromeric region. Females X₁X₁ and X₁X₂ exhibited repetitive DNA in telomeric regions which contributed to their morphological differentiation, while the Y chromosome evidenced the accumulation of specific and differential repetitive sequences. In contrast, autosomes showed balanced signal with no sex-chromosome-specific sequence accumulation.

Conclusions: Our findings highlight the role of repetitive DNA in the differentiation and evolution of sex chromosomes in A. fraterculus sp. 1, shedding new light on the origin and diversification of Anastrepha species in South America and providing valuable cytogenetic insights for future research.

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.