A Novel Biosensor for Highly Sensitive DNA Damage Detection Using TdT and CRISPR-Cas12a

Abstract

This study presents a highly sensitive fluorescence biosensor integrating TDT enzyme and CRISPR-Cas12a for precise detection of sperm DNA damage, achieving a detection limit of 0.99 pM and a linear range of 0.001–0.2 nM. Its exceptional sensitivity, simplicity, and versatility make it a transformative tool for reproductive medicine and clinical diagnostics.