Optimization of culturing conditions for leafcutter ant associated, antimicrobial producing Pseudonocardia by adding ants´ cuticular hydrocarbons

IF 1.6 3区环境科学与生态学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemoecology Pub Date : 2024-11-22 DOI:10.1007/s00049-024-00414-5

Johannes-Paul Fladerer-Grollitsch, Juliana Fitzek, Kristina M. Sefc, Franz Bucar

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引用次数: 0

Abstract

The purpose of the present study was to investigate the influence of cultivation parameters on the growth of leafcutter ant associated actinomycete Pseudonocardia sp. These bacteria live in a very specialized symbiosis with the leafcutter ant Acromyrmex octospinosus and fungi of the genus Leucoagaricus. Pseudonocardia are known to produce antibiotics but are very hard to grow as a result of the complexity of their symbiosis. The bacteria were grown on yeast-malt-extract agar and the influence of pH, temperature conditions and the addition of chitin and a cuticular hydrocarbon (CHC) solution specific to leafcutter ants on the growth of Pseudonocardia and other bacteria inhabiting the leafcutter ants´ surface was tested. The best growth rate of Pseudonocardia was achieved by cultivation at 28 °C in a medium with pH 7 supplemented with chitin and CHC solution. Additionally, these conditions inhibited the growth of other competitive bacteria. This is the first report on the optimization of growth media for leafcutter ant associated Pseudonocardia adjusted to the conditions found on the ants´ cuticle.

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添加蚂蚁表皮碳氢化合物对切叶蚁伴生抗菌假心菌培养条件的优化

本研究的目的是研究栽培参数对切叶蚁伴生放线菌Pseudonocardia sp.生长的影响。这些放线菌与切叶蚁Acromyrmex octospinosus和Leucoagaricus属真菌生活在一个非常特殊的共生关系中。众所周知，假心菌可以产生抗生素，但由于其共生关系的复杂性，很难生长。在酵母-麦芽提取物琼脂上培养细菌，考察pH、温度条件以及添加几丁质和切叶蚁专用的表皮烃（CHC）溶液对切叶蚁表面假心菌和其他细菌生长的影响。在pH为7、添加甲壳素和CHC溶液的培养基中，在28℃条件下培养，获得了最佳的生长速率。此外，这些条件抑制了其他竞争细菌的生长。本文首次报道了根据切叶蚁角质层的生长条件，对切叶蚁相关假心的生长培养基进行了优化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemoecology 环境科学-生化与分子生物学

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： It is the aim of Chemoecology to promote and stimulate basic science in the field of chemical ecology by publishing research papers that integrate evolution and/or ecology and chemistry in an attempt to increase our understanding of the biological significance of natural products. Its scopes cover the evolutionary biology, mechanisms and chemistry of biotic interactions and the evolution and synthesis of the underlying natural products. Manuscripts on the evolution and ecology of trophic relationships, intra- and interspecific communication, competition, and other kinds of chemical communication in all types of organismic interactions will be considered suitable for publication. Ecological studies of trophic interactions will be considered also if they are based on the information of the transmission of natural products (e.g. fatty acids) through the food-chain. Chemoecology further publishes papers that relate to the evolution and ecology of interactions mediated by non-volatile compounds (e.g. adhesive secretions). Mechanistic approaches may include the identification, biosynthesis and metabolism of substances that carry information and the elucidation of receptor- and transduction systems using physiological, biochemical and molecular techniques. Papers describing the structure and functional morphology of organs involved in chemical communication will also be considered.