Application of ATR-Fourier transform infrared spectroscopy in fast and simultaneous determination of leaf chemical and functional properties of forest herb species

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-02-13 DOI:10.1016/j.talanta.2025.127738

Tomasz Durak , Beata Ciak , Roma Durak , Joanna Depciuch

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引用次数: 0

Abstract

ATR-Fourier transform infrared spectroscopy was used to determine the carbon and nitrogen content in the leaves of herbaceous forest plant species and functional traits associated with the leaf economic spectrum (one of the two-dimensional global spectrum of plant form and function), and monitoring plant physiological status under elevated temperature conditions. The content of carbon and nitrogen determined by traditional methods validated the accuracy of ATR-FTIR method. It was also shown that in the case of forest herbs, the ATR-FTIR method is an efficient tool for determining functional traits (such as specific leaf area (SLA) and leaf dry matter content (LDMC)) related to the leaf economics spectrum, and to diagnose the photophysiological state of plants after changes of temperature (changes of day/night temperature from 21/13 °C to 25/17 °C). Measuring the areas of three absorption bands of the ATR-FTIR spectra related to amides I and II (between 1700 cm⁻¹ and 1500 cm⁻¹), carbohydrates (cellulose and hemicellulose; between 1200 cm⁻¹ - 850 cm⁻¹) and amide III (between 1290 cm⁻¹ and 1190 cm-¹) allowed for determination of all analysed chemical and functional properties of leaves. Based on selected absorption bands accurately estimated C and N content, with coefficients of correlation (r) of 0.88 for C and 0.84 for N. SLA and LDMC were also predicted, with r values of 0.88 and −0.91, respectively. Moreover, ATR-FTIR proved to be a rapid, non-destructive tool for monitoring the plant physiological status, as demonstrated by the significant correlation (r = 0.99) between the chlorophyll fluorescence performance index (PI) and ATR-FTIR data. ATR-FTIR has been demonstrated as an efficient tool for simultaneous quantification of leaf carbon and nitrogen content, economic functional traits, and physiological status of forest herb plants.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.