Resveratrol affects proliferation, apoptosis and migration of fibroblast cells as well as inhibits COL1A1/2 and TGFB2/3 gene expression

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2025-09-02 DOI:10.1080/10286020.2025.2462610

Mei-Li Li , Kun Pang , Lei Qiao , Zhi-Qiang Guan , Chao-Ju Gong , Song-Tao Li , Zhi-Ming Tang , Yi-Ling Zhang , Ji-Cun Ding

引用次数: 0

Abstract

Primary fibroblasts isolated from pterygium and skin pathological scar were identified by immunofluorescence staining. It was found by CCK8 and TUNEL that high-dose resveratrol, a Chinese medicine monomer, could inhibit proliferation, promote apoptosis and restrain migration. Explored by whole-transcriptome sequencing and LC-MS/MS, 1531 up-regulated mRNAs, 1022 down-regulated mRNAs, 79 up-regulated proteins and 84 down-regulated proteins were identified in resveratrol-treated pterygium fibroblasts. It was shown by KEGG, GO, PPI and the following bioinformatics analysis that resveratrol may affect proliferation, apoptosis and migration of fibroblast through TGFB2/3 and COL1A1/2.

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白藜芦醇影响成纤维细胞的增殖、凋亡和迁移，抑制COL1A1/2和TGFB2/3基因的表达。

采用免疫荧光染色法对分离自翼状胬肉和皮肤病理瘢痕的原代成纤维细胞进行鉴定。CCK8和TUNEL实验发现，大剂量的中药单体白藜芦醇具有抑制细胞增殖、促进细胞凋亡和抑制迁移的作用。通过全转录组测序和LC-MS/MS，在白藜芦醇处理的翼状胬肉成纤维细胞中鉴定出1531个上调mrna， 1022个下调mrna， 79个上调蛋白和84个下调蛋白。KEGG、GO、PPI和随后的生物信息学分析表明，白藜芦醇可能通过TGFB2/3和COL1A1/2影响成纤维细胞的增殖、凋亡和迁移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.