The neuroprotective effect of long non-coding RNA TINCR against cerebral ischemia-reperfusion injury via targeting miR-125b-5p.

IF 1.5 4区医学 Q4 NEUROSCIENCES

Folia neuropathologica Pub Date : 2024-01-01 DOI:10.5114/fn.2023.133134

Lifang Guo, Panpan Wu, Rongjiao Zhou, Hongyan Wang, Meng Li, Haitao Zhang, Deyong Du

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引用次数: 0

Abstract

Introduction: Cerebral ischemia-reperfusion (CI/R) injury commonly occurs in ischemic stroke (IS) patients. The study examined the role of long non-coding RNA (lncRNA) TINCR in a middle cerebral artery occlusion and reperfusion (MCAO/R) induced rat model and oxygen-glucose deprivation/re-oxygenation (OGD/R) induced neuron cell models.

Material and methods: Rats were treated with MCAO/R to induce IS animal models and neural stem cells (NSCs) were treated with OGD/R to establish cell models. The neurological function, cerebral infarction area, and inflammation of rats were evaluated. Cell proliferation, migration and apoptosis were assessed. Target association between TINCR and miR-125b-5p was verified. Based on the competing endogenous RNA (ceRNA) regulatory network, the rescue experiments were done in NSCs via cell transfection.

Results: In MCAO/R rats, downregulated expression of lncRNA TINCR was tested, accompanied by neurological dysfunction and cerebral infarction. TINCR overexpression in rats led to the recovery of neurological dysfunction and cerebral infarction, while inflammation and apoptosis were inhibited. In accordance with in vivo experiment results, declined TINCR was also tested in OGD/R treated NSCs. The rescue experiments demonstrated that TINCR overexpression promoted NSC proliferation and migration, but suppressed cell apoptosis and inflammation. TINCR serves as a ceRNA of miR-125b-5p, and miR-125b-5p abolished the protective role of TINCR in OGD/R cell models.

Conclusions: LncRNA TINCR attenuated CI/R injury through competitively binding to miR-125b-5p.

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来源期刊

Folia neuropathologica 医学-病理学

CiteScore

2.50

自引率

5.00%

发文量

审稿时长

>12 weeks

期刊介绍： Folia Neuropathologica is an official journal of the Mossakowski Medical Research Centre Polish Academy of Sciences and the Polish Association of Neuropathologists. The journal publishes original articles and reviews that deal with all aspects of clinical and experimental neuropathology and related fields of neuroscience research. The scope of journal includes surgical and experimental pathomorphology, ultrastructure, immunohistochemistry, biochemistry and molecular biology of the nervous tissue. Papers on surgical neuropathology and neuroimaging are also welcome. The reports in other fields relevant to the understanding of human neuropathology might be considered.