Small Arteries From Old Spontaneously Hypertensive Rats Exhibit Enhanced Endothelium-Independent Vasodilatory Capacity and Reduced Stiffness

IF 1.9 4区医学 Q3 HEMATOLOGY

Microcirculation Pub Date : 2025-02-17 DOI:10.1111/micc.70004

Francisco I. Ramirez-Perez, Thomas J. Jurrissen, Marc A. Augenreich, Jorge A. Castorena-Gonzalez, Mariana Morales-Quinones, Christopher A. Foote, Zahra Nourian, Olubodun M. Lateef, Natnicha Imkaew, Zhe Sun, Michael A. Hill, Gerald A. Meininger, Jaume Padilla, Luis A. Martinez-Lemus

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引用次数: 0

Abstract

Objective

In conduit arteries, aging and hypertension are associated with stiffening characterized by increased cytoskeletal F-actin and endothelial dysfunction. Herein, we determined if this also happens at the level of the resistance vasculature.

Methods

We retrospectively compared the mechanical and structural characteristics of small arteries isolated from older hypertensive and younger normotensive (64.7 ± 2.8 vs. 32.1 ± 1.9 years old) human subjects. The intersection of aging and hypertension was studied in small mesenteric arteries from old (88 weeks of age) spontaneously hypertensive (SHR) and Wistar Kyoto (WKY) normotensive rats.

Results

Arteries from older hypertensive subjects were stiffer and had more F-actin, relative to those from younger normotensives. Comparatively, arteries from old SHRs showed reduced stiffness and increased vasodilation to sodium nitroprusside without changes in F-actin. Matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) were increased in the SHR arteries and exposure of naive arteries to exogenous MMP-2 and MMP-9 augmented responsiveness to sodium nitroprusside and adenosine.

Conclusions

In conclusion, resistance arteries from old SHRs are softer and vasodilate more to exogenous nitric oxide than those of WKY rats. This improved endothelial-independent vasodilation is associated with an increased vascular expression of MMP-2 and MMP-9. We further conclude that aging and hypertension effects on the microcirculation may vary between species and vascular beds.

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.