Identification and genetic characterization of Xiabuqu River virus: A novel member of the Iflaviridae family detected in soft ticks from Tibet, China

IF 2.5 4区医学 Q3 VIROLOGY

Virus research Pub Date : 2025-02-20 DOI:10.1016/j.virusres.2025.199543

Meixi Ren , Mingli Sun , bataj Dan , Yingxin Tu , Guoyu Niu , Weiping Lin , Hengyi Sun , Yujing Zhu

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引用次数: 0

Abstract

Soft ticks, an important group of blood-sucking arthropods in nature, are widely distributed globally and can carry a wide range of pathogens, including Theileria ovis, Anaplasma ovis, Rickettsia spp. and African swine fever virus. In this study, we identified a novel single positive-stranded RNA virus, tentatively named Xiabuqu River virus (XRV), for the first time from soft ticks collected in Shigatse, Tibet. A total of 96 engorged soft ticks were collected from Tibetan sheep, with each tick assigned to a separate pool for analysis. The complete coding sequence of XRV was determined through next-generation sequencing, revealing a sequence length of 9277 nucleotides that includes a single open reading frame (ORF) encoding proteins such as the capsid protein, RNA helicase, and RNA-dependent RNA polymerase (RDRP). Quantitative RT-PCR and nested PCR were utilized to investigate the distribution of XRV within the tick sample. Pairwise distance analysis revealed that all obtained viral sequences shared a high nucleotide identity. Phylogenetic analysis demonstrated that XRV clustered with Lhasa Iflav tick virus 1, Fuyun tick virus 2, and Hubei tick virus 2. Further analyses indicated that XRV is a new member of the unclassified genus Iflavirus within the family Iflaviridae.

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来源期刊

Virus research 医学-病毒学

CiteScore

9.50

自引率

2.00%

发文量

239

审稿时长

43 days

期刊介绍： Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.