Structure of T5-Like Phage dUTPase from Vibrio Phage Phi3

IF 0.6 4区 材料科学 Q4 CRYSTALLOGRAPHY
A. S. Glukhov, U. F. Dzhus, I. A. Kolyadenko, G. K. Selikhanov, A. G. Gabdulkhakov
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引用次数: 0

Abstract

The first crystal structure of dUTPase from the bacteriophage Vibrio phage phi3 was determined at 1.95 Å resolution. This protein has a homotrimeric quaternary structure characteristic of this type of dUTPases and contains a short loop in the N-terminal region of the polypeptide chain, which is specific for Т5-like bacteriophage dUTPases. It was demonstrated that, despite a low amino acid sequence homology between phage (Т5 and phi3) dUTPases, phi3 dUTPase can effectively replace phage Т5 dUTPase during the lytic cycle.

Abstract Image

噬菌体Vibrio Phage Phi3中t5样噬菌体dUTPase的结构
以1.95 Å的分辨率测定了噬菌体Vibrio phage phi3中dUTPase的第一个晶体结构。该蛋白具有这类dutpase特有的同三聚体四级结构,在多肽链的n端区域含有一个短环,这是Т5-like噬菌体dUTPases所特有的。结果表明,尽管噬菌体(Т5)和phi3 dUTPase之间的氨基酸序列同源性较低,但phi3 dUTPase可以在裂解周期中有效地取代噬菌体Т5 dUTPase。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Crystallography Reports
Crystallography Reports 化学-晶体学
CiteScore
1.10
自引率
28.60%
发文量
96
审稿时长
4-8 weeks
期刊介绍: Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.
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